ISSN:
1573-5087
Keywords:
sterilisation
;
culture
;
contamination
;
micropropagation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Abstract The effectiveness of Sodium dichloroisocyanurate as a disinfectant for micropropagated plants was assessed. Analysis of the microbial flora of micropropagated plants showed a wide range of bacteria with predominantly Pseudomonas, Xanthomonas and Actinomycetes. Sodium dichloroisocyanurate was highly stable both as preprepared tablets and as solutions maintained at room temperature. Sterilisation of a range of plants which were heavily contaminated with bacteria was examined. Phytotoxicity was generally low and restricted to old leaves and cut surfaces. Solutions of Sodium dichloroisocyanurate were more effective at high concentrations (5000 ppm) than a commercially available bleach for disinfection of shoot cultures. Sodium dichloroisocyanurate was also used at low concentrations (300 ppm) for longer periods (24 h–48 h) to disinfect shoot explants from the field, and was at least as effective for sterilisation as a combination of Mercuric Chloride and Calcium hypochlorite.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00024060
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