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  • DNA loop anchorage sites  (1)
  • competitive polymerase chain reaction  (1)
Collection
Keywords
Publisher
Years
  • 1
    Electronic Resource
    Electronic Resource
    The quest for a humanori (1994)
    Springer
    Genetica 94 (1994), S. 255-266 
    Details
    ISSN: 1573-6857
    Keywords: DNA replication ; origin ; competitive polymerase chain reaction ; transcription ; lamin B2
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Attempts at identifying DNA replication origins in human cells have been performed with a variety of molecular genetic and biochemical approaches, with often controversial results. The combination of bromodeoxyuridine labelling, immunopurification of newly synthesized labelled DNA, measurement of the relative abundance of markers in this DNA by quantitative competitive PCR, has allowed the identification within 450 bp of the start-site of DNA replication located at the human lamin B2 gene. The origin is located near the non-transcribed spacer between two highly transcribed genes and shows evidence of a number of specific protein-DNA interactions, the most prominent of which disappears when the cells are differentiated into a non-proliferating state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01443439
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    DNA loop anchorage region colocalizes with the replication origin located downstream to the human gene encoding lamin B2 (1998)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 69 (1998), S. 13-18 
    Details
    ISSN: 0730-2312
    Keywords: nuclear matrix ; replication origin ; topoisomerase II-mediated DNA loop excision ; DNA loop anchorage sites ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: The recently developed procedure of topoisomerase II-mediated DNA loop excision has been used to analyze the topological organization of a human genome fragment containing the gene encoding lamin B2 and the ppv1 gene. A 3.5 kb long DNA loop anchorage/topoisomerase II cleavage region was found within the area under study. This region includes the end of the lamin B2 coding unit and an intergenic region where an origin of DNA replication was previously found. These observations further corroborate the hypothesis that DNA replication origins are located at or close to DNA loop anchorage regions. J. Cell. Biochem. 69:13-18, 1998. © 1998 Wiley-Liss, Inc.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
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