ISSN:
1573-5060
Keywords:
chromosome number
;
embryo rescue
;
flow cytometry
;
interspecific hybrid
;
Limonium perezii
;
Limonium sinuatum
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Abstract Interspecific hybrids between Limonium perezii and L. sinuatum were obtained using L. perezii as the female parent. The embryos which formed were rescued by culturing 12- to 16-day-old embryos within their ovules. The ovules containing the embryos were cultured on modified B5 medium and the embryos were later excised and re-plated onto fresh medium. The embryos grew for varying lengths of time eventually reaching lengths of up to 10 mm, before ceasing growth. At this point they died. Transfer to modified MS medium containing 3 mg l-1 TDZ for 24 hours induced callus growth in the embryo derived tissues from which shoots developed. These shoots were proliferated on modified MS medium containing IBA, BAP and GA3. Shoots transferred to modified MS medium with added IBA for 6 days initiated roots which continued development after transfer of the explants to modified growth-regulator-free MS medium. After a further 28 days the plantlets were transferred to soil. The hybrid nature of one of the embryo-derived plants was determined by flow cytometry and by examination of morphological features. The mean DNA contents of 2C nuclei from L. perezii, the hybrid and L. sinuatum were 8.69 pg, 7.59 pg and 6.42 pg, respectively. Mitotic and meiotic chromosome counts from L. perezii and L. sinuatum and their hybrid revealed that they had chromosome numbers of 14, 16 and 15, respectively. Morphological analyses of leaves and flowers showed that the hybrid displayed a number of features intermediate between both parents.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1018384329409
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