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  • 1
    ISSN: 1617-4623
    Keywords: General amino acid permease ; Protein kinase ; Serine-rich protein ; Transport protein ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary TheNPR1 gene ofSaccharomyces cerevisiae plays a central role in controlling permease activity; its product is required to promote the activity of at least six distinct transport systems for nitrogenous nutrients under conditions of nitrogen catabolite derepression. We report here the nucleotide sequence of the clonedNPR1 gene. The predicted amino acid sequence indicates thatNPR1 encodes a protein of 86 kDa which appears to be organized into two distinct structural domains. The amino-terminal domain of NPR1 (residues 1 to 440) contains 26% serine residues and several regions strongly enriched for PEST residues suggesting a short half-life for the NPR1 protein. The carboxy-terminal region of NPR1 contains consensus sequences characteristic of the catalytic domains of protein kinases. Therefore, NPR1-dependent positive control of nitrogen transport systems most likely involves protein phosphorylation. Northern analysis indicates that the absence of general amino acid permease (GAP1) activity innpr1 mutants is not due to reduction in transcription or messenger stability. Hence, the NPR1 protein probably acts at the post-transcriptional level. Proteins that may serve as substrates for phosphorylation are discussed.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0749-503X
    Keywords: Saccharomyces cerevisiae ; chromosome XI ; protein kinase ; sequencing project ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We have completely sequenced on both strands a continuous DNA segment of 36·2 kb located on the left arm of Saccharomyces cerevisiae chromosome XI. Sequence analysis reveals the presence of 20 open reading frames (ORFs) at least 100 amino acids long. Five of these ORFs correspond to known genes; five others show homology with known proteins; the ten remaining ORFs identified show no detectable homology with other protein sequences contained in data banks and may represent new biological functions. The sequence has been deposited in the EMBL data library under Agreement Number Z26877.
    Additional Material: 2 Ill.
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  • 3
    ISSN: 0749-503X
    Keywords: Saccharomyces cerevisiae ; chromosome XI ; DNA sequencing project ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: A 20·5 kb DNA fragment from the left arm of chromosome XI of Saccharomyces cerevisiae has been sequenced and analysed. Thirteen open reading frames (ORFs) for proteins longer than 100 amino acids were discovered. Among them, two are the known genes MRP49 and TPK3; two others encode proteins which show strong similarity with a yeast putative protein kinase and a yeast choline transport protein; one other shows weaker similarity with a yeast Ca2+/calmodulin-dependent protein kinase. Moreover, two putative proteins encoded by ORFs located in the sequenced fragment are closely similar to non-yeast proteins: the Caenorhabditis elegans elongation factor 2 and a glutamic acid-rich protein of Plasmodium falciparum. The sequence has been deposited in the EMBL data library under Accession Number Z26878.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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