ISSN:
1573-0778
Keywords:
hepatoma
;
cell culture
;
microcarrier
;
agitation rate
;
cell inoculation density
;
growth rate
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract Hepatoma cells, HepG2, grew normally on microcarriers even at a relatively high agitation rate if sufficient time was allowed for cell attachment and adhesion. However, if a high agitation rate was applied shortly after initial cell attachment, the growth rate was retarded. This sensitivity to mechanical agitation appears to be dependent on the inoculation cell density.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00386146
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