ISSN:
0730-2312
Keywords:
methylation
;
DNA
;
chromosomes 11p and 17 p13.3
;
human breast adenocarcinoma
;
human colon adenocarcinoma
;
γ-globin
;
Ha-ras
;
parathyroid hormone
;
catalase
;
calcitonin
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
Notes:
In breast and colon adenocarcinomas methylation patterns at CCGG sites of several loci located on the short arm of chromosome 11 were determined by Southern blot analysis. Results obtained indicate that all tumor samples (20/20) exihibit DNA methylation changes when compared to their normal counterparts. In colon tumors,i-globin gene is usually hypomethylated (9/10), whereas Ha-ras gene, which is located in the same region, retains an unmodified DNA methylation pattern. Hypomethylation of parathyroid hormone (5/10) and catalase genes (4/10) are also frequently detected in colon tumor specimens. For the catalase gene the region around exon is the only one which is affected by these changes. In breast adenocacinoma, modifications of the methylation patterns are less frequently observed. However, hypomethylation of the i-globin gene is a very common event in these tumors (8/10), and it is also detected (2/2) in lobular carcinoma in situ which is an early step in breast tumorgenesis. In addition, hypermethylation of a CpG island is also observed at the locus 17p13.3 in both colon (5/5) and breast (4/9) adenocarcinomas. In the tumoral tissues which containj a limited amount of CpG. Some of these alterations seen, therefore, to be tumor and sequence specific.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jcb.240560113
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