ISSN:
1040-7685
Keywords:
capillary isotachophoresis
;
determination of pyruvate
;
acetoacetate
;
3-hydroxybutyrate
;
lactate
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
An isotachophoretic method was developed and optimized for simultaneous determination of pyruvate, acetoacetate, lactate, and 3-hydroxybutyrate in 1-10 μL of untreated heparin plasma of patients with diabetes mellitus. A commercial instrument with a column switching system was used. The preseparation capillary was filled with the leading electrolyte of pH 4.2 and the analytical capillary was filled with leading electrolyte of pH 3.0. The concentrations of individual ketone bodies and lactate found in human plasma varied within 0.01-1.4 mmol/L and 0.8-11.8 mmol/L, respectively. Analysis time was about 30 min.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/mcs.1220020207
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