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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Methods in cell science 22 (2000), S. 217-223 
    ISSN: 1573-0603
    Keywords: Flow cytometry ; Muscle cell ; Porcine ; Transfection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A detailed methodology is described for fluorescence-activated cell sorting (FACS) of porcine muscle cells that have been transfected to express green fluorescent protein (GFP). Cells are liberated from porcine skeletal muscle and primary cultures are transfected with DNA encoding GFP. Primary cultures are subjected to immunocytochemistry using a primary muscle-specific monoclonal antibody followed by incubation with a phycoerythrin-conjugated second antibody. Transfected myoblasts aresorted from fibroblasts using forward angle light scatter and ninety degree light scatter, phycoerythrin fluorescence, and GFP fluorescence. These procedures allow for isolation of genetically-engineered porcine muscle cells more rapidly than traditional clonal selection procedures. Consequently, FACS provides porcine myoblast populations that retain the majority of their replicative capacity and are not contaminated with non-myogenic cells.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Experimental mechanics 40 (2000), S. 75-82 
    ISSN: 1741-2765
    Keywords: residual stress ; aluminum alloy ; forging ; solution heat treatment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Precipitation-hardened aluminum alloys gain their high strength through heat treatment involving a severe quenching operation, which can have the adverse effect of introducing residual stresses. The finite element code ABAQUS is used to simulate the quenching of aluminum alloy 7010 in an attempt to predict the residual stress distribution that develops in simple shapes. The rate of heat transfer from the material is determined using the finite element method to predict the heat transfer coefficient from surface cooling curves achieved experimentally. The flow stress of the material is assumed to be strain rate dependent and to behave in a perfectly plastic manner. The predicted residual stress magnitudes and directions are compared to values determined using the holedrilling strain gage method and the X-ray diffraction technique.
    Type of Medium: Electronic Resource
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