ISSN:
1420-9071
Keywords:
Platelets
;
acridine organge
;
storage granules
;
fluorescence quenching
;
accumulation ability
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary A new approach to the evaluation of the uptake of fluorescent, probes by intact, cells is described. Acridine organe (AO) was used because it can be selectively accumulated by serotonin-containing granules of platelets. Analysis of the fluorescence signal allows the estimation of the relative volume of the granules and the equilibrium coefficients for AO transport across the cytoplasm and granule membranes. The following results were obtained for human and rabbit platelets: the relative volumes of the granules were 14±1% and 29±2%, the ratios of intragranular-extracellular probe concentration were 2260±382 and 30,000±5500, and the cytoplasm-extracellular medium concentration ratios were 375±60 and 225±60, respectively.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01953316
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