ISSN:
1617-4623
Keywords:
Antibiotic resistance
;
Gentamicin acetyltransferase
;
aacC nucleotide sequence
;
Transposon Tn21
;
Plasmid evolution
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The aminoglycoside-3-O-acetyltransferase-I gene (aacC1) from R plasmids of two incompatibility groups (R1033 [Tn1696], and R135) was cloned and sequenced. In the case of R1033, it was shown that theaacC gene is coded by a precise insertion of 833 bp between theaadA promoter and its structural gene in a Tn21 related transposon (Tn1696). This insertion occurs at the same target sequence as that of the OXA-1 β-lactamase gene insertion in Tn2603. Upstream of theaacC gene, we found an open reading frame (ORF) which is probably implicated in the site-specific recombinational events involved in the evolution of this family of genetic elements. These results provide additional confirmation of the role of Tn21 elements as naturally occurring interspecific transposition and expression casssettes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02464882
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