ISSN:
1612-1112
Keywords:
HPLC methods
;
Mycotoxins
;
Zearlenone
;
Vomitoxin (Deoxynivalenol)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary HPLC procedures for analyses of the fusariotoxins zearalenone and vomitoxin in individual food- and feedstuffs as well as in mixed feed are described. Zearalenone is separated on a column with polar stationary phase (25 cm × 4.6 mm i.d., particle size 7 μm), eluted with a chloroform-isooctane (75/25, v/v)+1.5% methanol mixture and detected fluorometrically. The quantitative determination was possible in all analyzed samples with a detection limit of 2μg/kg with 70–80% recovery. Vomitoxin is fractionated by HPLC (C 18 1 column, 25 cm×4 mm i.d., 5 μm particle size) with water-methanol (60/40, v/v) mobile phase and determined by combining GLC or TLC with UV detection. The detection limit in mixed feed with interfering substances was 25 μg/kg (recovery 25–35%). The separation by HPLC makes preparation of pure vomitoxin possible. The described methods are fast, simple and low cost and are suitable for routine analyses.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02261528
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