ISSN:
1432-0983
Schlagwort(e):
Keywords Kluyveromyces marxianus
;
Yeast
;
HIS3
;
Mutants
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract A method to obtain K. marxianus mutants has been developed. Different auxotrophic mutants were isolated by nystatin and snail-enzyme enrichment procedures using an incubation time of 2 h before adding the antibiotic or the enzyme respectively. All his mutants analyzed by complementation tests turned out to belong to the same complementation group. Some of them were transformed and complemented by the S. cerevisiae HIS3 gene. These non-reverting his3 mutants contain no heterologous sequence, which is essential to make them acceptable for application in the food industry.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/s002940050105
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