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  • Yeast  (18)
  • DNA repair  (10)
  • Springer  (28)
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  • 1
    Electronic Resource
    Electronic Resource
    Overexpression ofADH1 confers hyper-resistance to formaldehyde inSaccharomyces cerevisiae (1996)
    Springer
    Current genetics 29 (1996), S. 437-440 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Formaldehyde ; Hyper-resistance ; Alcohol dehydrogenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In an attempt to clone genes involved in resistance to formaldehyde we have screened a genomic library based on the episomal plasmid YEp24 for the ability to increase resistance to formaldehyde in a wild-type strain. In addition toSFA, the gene encoding the formaldehyde dehydrogenase Adh5, an enzyme most potent in formaldehyde de-toxification, we isolated a second plasmid that conferred a less pronounced but significant hyper-resistance to formaldehyde. Its passenger DNA contained the geneADH1, encoding alcohol dehydrogenase 1 (EC 1.1.1.1), which could be shown to be responsible for the observed hyper-resistance phenotype. Construction of anadh1-0 mutant revealed that yeast lacking a functionalADH1 gene is sensitive to formaldehyde. While glutathione is essential for Adh5-mediated formaldehyde de-toxification, Adh1 reduced formaldehyde best in the absence of this thiol compound. Evidence is presented that formaldehyde is a substrate for Adh1 in vivo and in vitro and that its cellular de-toxification employs a reductive step that may yield methanol.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02221511
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  • 2
    Electronic Resource
    Electronic Resource
    Overexpression of the SNQ3/YAP1 gene confers hyper-resistance to nitrosoguanidine in Saccharomyces cerevisiae via a glutathione-independent mechanism (1994)
    Springer
    Current genetics 25 (1994), S. 469-471 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; GSH ; DNA alkylation ; MNNG
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The MNNG hyper-resistance of yeast transformants containing multiple copies of the SNQ3/YAP1 yeast gene is not caused by lowered MNNG activation due to depleted pools of glutathione. On the contrary, the SNQ3/YAP1-encoded protein stimulates production of GSH, apparently by promoter activation due to the AP-1 recognition element. Expression of at least one further gene, encoding a protein with a strong detoxifying activity, must also be stimulated to explain the MNNG hyper-resistance phenotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00351788
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  • 3
    Electronic Resource
    Electronic Resource
    A ten-minute protocol for transforming Saccharomyces cerevisiae by electroporation (1992)
    Springer
    Current genetics 22 (1992), S. 335-336 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Rapid transformation ; Cell age
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We present a simplified and rapid method for the transformation of yeast cells by electroporation. Stationary cells, scraped off the agar of Petri dish cultures stored in the refrigerator for up to 6 weeks, are suspended in sorbitol buffer, spun down by gentle centrifugation, transferred into the electroporation cuvette, and immediately subjected to transformation via electroporation. Transformation efficiency of this 10-min method, which does not require the preparation of cell cultures, is about 10% of the hitherto best performing transformation procedure using cells of defined growth phase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00317931
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  • 4
    Electronic Resource
    Electronic Resource
    Formation and stability of interstrand cross-links induced by cis- and trans-diamminedichloroplatinum (II) in the DNA of Saccharomyces cerevisiae strains differing in repair capacity (1989)
    Springer
    Current genetics 16 (1989), S. 331-338 
    Details
    ISSN: 1432-0983
    Keywords: Platinum compounds ; Yeast ; Repair mutants ; Interstrand cross-links ; DNA degradation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Four haploid yeast strains differing in proficiency for DNA repair were treated with cis- or transDDP. The wild type was least sensitive while the excision-deficient mutants rad1, rad2 and snm1exhibited higher sensitivities to either platinum compound. In all four strains tested cisDDP showed a two- to five-fold higher cytotoxicity than equimolar concentrations of transDDP. DNA interstrand cross-linking was caused by both agents in all strains. However, transDDP introduced more DNA cross-links at exposure times up to 6 h while cisDDP was the more active cross-linking agent at longer times. There was no clear-cut correlation of the number of DNA interstrand cross-links with survival. Formaldehyde-treated cells showed DNA with lower buoyant density due to proteinase K sensitive DNA-protein cross-linking; this effect was not observed after treatment with either platinum compound. Post-treatment incubation of wild-type cells exposed to cisDDP led to degradation of DNA by single and double-strand breaks, parallel with further increase of DNA interstrand cross-linking. DNA from transDDP-treated cells did not show extensive degradation although interstrand cross-links were lost during liquid holding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00340711
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  • 5
    Electronic Resource
    Electronic Resource
    Mutant Gene snm2-1 ts, conferring thermoconditional mutagen sensitivity in Saccharomyces cerevisiae, is allelic with RAD5 (1982)
    Springer
    Current genetics 5 (1982), S. 93-95 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Thermoconditional DNA repair ; Mutagenesis ; Allelism test
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Of two mutant genes (snm1-2 ts and snm2-1 ts) conferring thermoconditional mutagen sensitivity in Saccharomyces cerevisiae one (snm2-1 ts) is shown to be centromere-linked. At the restrictive temperature this allele reduces UV-induced back mutation frequency of the ochre allele hiss-2 but has no influence on forward mutation at the CAN1 locus. Complementation tests and recombination analysis revealed snm2 ts to be allelic with rad5 (rev2).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00365699
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  • 6
    Electronic Resource
    Electronic Resource
    The RAD50 gene of Saccharomyces cerevisiae is not essential for vegetative growth (1986)
    Springer
    Current genetics 10 (1986), S. 487-489 
    Details
    ISSN: 1432-0983
    Keywords: DNA repair ; Gene disruption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The gene RAD50 was located by the ability of subclones to restore the Rad+ phenotype following transformation of a rad50-1 mutant. Disruption of the gene was achieved by directed integration of a plasmid carrying a fragment internal to RAD50. Haploids with the disrupted gene are viable and do not differ in growth rate or plating efficiency from isogenic rad50-1 or Rad+ strains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419878
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  • 7
    Electronic Resource
    Electronic Resource
    Yeast single copy gene URP1 is a homolog of rat ribosomal protein gene L21 (1993)
    Springer
    Current genetics 23 (1993), S. 15-18 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Rat ; Ribosomal protein ; 60S Ribosomal subunit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary This communication reports on a single-copy gene of Saccharomyces cerevisiae which is homologous to the rat ribosomal protein gene L21. The yeast and the rat genes show 59% identity in DNA sequences and in the predicted protein sequences. This yeast gene is, therefore, assumed to code for an as yet unassigned ribosomal protein (URP1). The URP1 open reading frame is 480 nucleotides long and can encode a protein of about Mr 18 200. Like most of the other known ribosomal protein genes, URP1 is interrupted by an intron in its 5′ terminal part and it is preceeded by upstream sequence elements which usually regulate transcription of these genes. Northern blot analysis reveals that the URP1 gene is actually expressed in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00336743
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  • 8
    Electronic Resource
    Electronic Resource
    Molecular characterisation of GTP1, a Saccharomyces cerevisiae gene encoding a small GTP-binding protein (1994)
    Springer
    Current genetics 26 (1994), S. 564-566 
    Details
    ISSN: 1432-0983
    Keywords: Small GTP-binding proteins ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract DNA sequence analysis upstream of the yeast DNA repair gene SNM1 revealed gene GTP1 with an ORF of 573 bp on chromosome XIII. The putative amino-acid sequence of the encoded protein shows homology to proteins of the ARF-class of small GTP-binding proteins. Homology within GTP-binding motifs is highly conserved. Gene disruption showed that GTP1 is not an essential gene and that it has no influence on the expression of the DNA repair gene SNM1 with which it shares a 191-bp promoter region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00309951
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  • 9
    Electronic Resource
    Electronic Resource
    Characterization of the role played by the RAD59 gene of Saccharomyces cerevisiae in ectopic recombination (1999)
    Springer
    Current genetics 36 (1999), S. 13-20 
    Details
    ISSN: 1432-0983
    Keywords: Key words Gene conversion ; DNA repair ; Ty elements ; Genomic instability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The RAD52 group of genes in the yeast Saccharomyces cerevisiae controls the repair of DNA damage by a recombinational mechanism. Despite the growing evidence for physical and biochemical interactions between the proteins of this repair group, mutations in individual genes show very different effects on various types of recombination. The RAD59 gene encodes a protein with similarity to Rad52p which plays a role in the repair of damage caused by ionizing radiation. In the present study we have examined the role played by the Rad59 protein in mitotic ectopic recombination and analyzed the genetic interactions with other members of the repair group. We found that Rad59p plays a role in ectopic gene conversion that depends on the presence of Rad52p but is independent of the function of the RecA homologue Rad51p and of the Rad57 protein. The RAD59 gene product also participates in the RAD1-dependent pathway of recombination between direct repeats. We propose that Rad59p may act in a salvage mechanism that operates when the Rad51 filament is not functional.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050467
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  • 10
    Electronic Resource
    Electronic Resource
    Hyper-resistance to nitrogen mustard in Saccharomyces cerevisiae is caused by defective choline transport (1991)
    Springer
    Current genetics 19 (1991), S. 423-427 
    Details
    ISSN: 1432-0983
    Keywords: Yeast ; Molecular cloning ; Nitrogen mustard hyper-resistance ; Choline transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The recessive hnm1 mutant allele is responsible for hyper-resistance to nitrogen mustard in Saccharomyces cerevisiae. Transformation with a single-copy HNM1 wild-type allele of such hyper-resistant mutants will restore wild-type sensitivity to nitrogen mustard. By contrast the presence of multi-copy vectors containing HNM1, in either a hyper-resistant hnm1 mutant or an HNM1 wild-type, will lead to a novel, mustard-sensitive phenotype unrelated to defects in DNA repair genes. Gene disruption of HNM1 revealed that this gene is nonessential for cells prototrophic for choline (CHO1) but lethal for cells with a cho1 genotype. Sensitivity to nitrogen mustard of wild-type HNM1, but not of hnm1 mutants, depends on the choline content of the growth medium, with cells grown in choline-free medium exhibiting the highest sensitivity. Sequencing of a 300 bp DNA fragment of HNM1 revealed the identity of this gene with the CTR locus, which is responsible for choline transport in Saccharomyces cerevisiae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00312732
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