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  • 1
    Electronic Resource
    Electronic Resource
    The Yarrowia lipolytica LEU2 gene (1987)
    Springer
    Current genetics 11 (1987), S. 377-383 
    Details
    ISSN: 1432-0983
    Keywords: Y. lipolytica ; LEU2 ; Yeast ; Leucine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A 2810 by DNA fragment containing the beta-isopropylmalate dehydrogenase gene of the dimorphic yeast Yarrowia lipolytica has been sequenced. The sequence contains an open reading frame of 405 codons, predicting a protein of 43,366 molecular weight. Protein sequence homology with the polypeptide encoded by the LEU2 gene of Saccharomyces cerevisiae is 64%, whereas DNA sequence homology is 61%. The 5′- and 3′-flanking regions of the Y. lipolytica LEU2 gene share only some general structural features common to genes of S. cereviside such as the presence and location of TATA boxes, CAAT boxes, CACACA repeats, the lack of G residues in the 5′-untranslated region and 3′-transcription terminators. Transcription of a 1.4 kb mRNA begins at a small cluster of sites approximately 40 base pairs before the initial ATG.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00378180
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  • 2
    Electronic Resource
    Electronic Resource
    Aromatic amino-acid biosynthesis in Candida albicans: identification of the ARO4 gene encoding a second DAHP synthase (1996)
    Springer
    Current genetics 29 (1996), S. 441-445 
    Details
    ISSN: 1432-0983
    Keywords: Key words  Aromatic amino-acid biosynthesis ; 3-deoxy-d-arabinoheptulosonate-7-phosphate synthase ; Aro3p/Aro4p ; 5-fluoroorotic acid ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   The primary step in the aromatic amino-acid biosynthetic pathway in Saccharomyces cerevisiae is catalyzed by two redundant isozymes of 3-deoxy-d-arabinoheptulosonate-7-phosphate (DAHP) synthase, either of which alone is sufficient to permit growth on synthetic complete media lacking aromatic acids (SC-Aro). The activity of one isozyme (encoded by the ARO3 gene) is feedback-inhibited by phenylalanine, whereas the activity of the other isozyme (encoded by the ARO4 gene) is feedback-inhibited by tyrosine. Transcription of both genes is controlled by GCN4. We previously cloned the ARO3 gene from the opportunistic pathogen Candida albicans and found that: (1) it can complement an aro3 aro4 double mutation in S. cerevisiae, an effect inhibited by excess phenylalanine; and (2)  its expression is induced in response to amino-acid deprivation, consistent with the presence of two putative GCN4-responsive promoter elements (Pereira and Livi 1993, 1995). To determine whether other DAHP synthases exist in C. albicans, we have constructed a homozygous aro3-deletion mutant strain. Such a mutant was found to be phenotypically Aro+, i.e., capable of normal growth on SC-Aro media, suggesting the presence of at least one additional isozyme. To confirm this result, a 222-bp DNA fragment was amplified by the polymerase chain reaction (PCR) from genomic DNA prepared from the homozygous aro3-deletion mutant, using a degenerate primer based on a conserved N-terminal region of Aro3p plus a degenerate comeback primer encoding a conserved region of the protein that lies within the deleted portion of the gene. The nucleotide sequence of this PCR fragment predicts a 74-amino acid DAHP synthase-related protein which shows strong homology to Aro3p from S. cerevisiae and C. albicans, but even greater homology (78% identity) to S. cerevisiae Aro4p. We conclude that cells of C. albicans contain a second Aro4p-related DAHP synthase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050069
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  • 3
    Electronic Resource
    Electronic Resource
    Aromatic amino-acid biosynthesis inCandida albicans: identification of theARO4 gene encoding a second DAHP synthase (1996)
    Springer
    Current genetics 29 (1996), S. 441-445 
    Details
    ISSN: 1432-0983
    Keywords: Aromatic amino-acid biosynthesis ; 3-deoxy-d-arabinoheptulosonate-7-phosphate synthase ; Aro3p/Aro4p ; 5-fluoroorotic acid ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The primary step in the aromatic amino-acid biosynthetic pathway inSaccharomyces cerevisiae is catalyzed by two redundant isozymes of 3-deoxy-d-arabino-heptulosonate-7-phosphate (DAHP) synthase, either of which alone is sufficient to permit growth on synthetic complete media lacking aromatic acids (SC-Aro). The activity of one isozyme (encoded by theARO3 gene) is feedback-inhibited by phenylalanine, whereas the activity of the other isozyme (encoded by theARO4 gene) is feedback-inhibited by tyrosine. Transcription of both genes is controlled by GCN4. We previously cloned theARO3 gene from the opportunistic pathogenCandida albicans and found that: (1) it can complement anaro3 aro4 double mutation inS. cerevisiae, an effect inhibited by excess phenylalanine; and (2) its expression is induced in response to amino-acid deprivation, consistent with the presence of two putative GCN4-responsive promoter elements (Pereira and Livi 1993, 1995). To determine whether other DAHP synthases exist inC. albicans, we have constructed a homozygousaro3-deletion mutant strain. Such a mutant was found to be phenotypically Aro+, i.e., capable of normal growth on SC-Aro media, suggesting the presence of at least one additional isozyme. To confirm this result, a 222-bp DNA fragment was amplified by the polymerase chain reaction (PCR) from genomic DNA prepared from the homozygousaro3-deletion mutant, using a degenerate primer based on a conserved N-terminal region of Aro3p plus a degenerate comeback primer encoding a conserved region of the protein that lies within the deleted portion of the gene. The nucleotide sequence of this PCR fragment predicts a 74-amino acid DAHP synthase-related protein which shows strong homology to Aro3p fromS. cerevisiae andC. albicans, but even greater homology (78% identity) toS. cerevisiae Aro4p. We conclude that cells ofC. albicans contain a second Aro4p-related DAHP synthase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02221512
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  • 4
    Electronic Resource
    Electronic Resource
    Genetic and environmental variation in anther, pollen and pistil dimensions in sesame (1996)
    Springer
    Sexual plant reproduction 9 (1996), S. 228-232 
    Details
    ISSN: 1432-2145
    Keywords: Key words Male transmission ; Anther dimensions ; Pollen dimensions ; Pistil dimensions ; Sesame ; Sesamum indicum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Measurements of anther (length, width, depth), pollen grain (percent fertility, polar diameter, equatorial diameter, polar diameter/equatorial diameter ratio, volume) and pistil (stigma length, style length, ovary length, total pistil length, stigma width, style width, ovary width) were taken on 12 diverse sesame (Sesamum indicum L.) genotypes on each of four collection dates in 1994. Highly significant differences among genotype means were obtained for all characters except polar diameter. Highly significant differences among environment (collection date) means were found for ten of the 15 characters measured. Highly significant geno- type × environment interactions were obtained for all characters except anther length. For the anther characters measured, relatively high repeatability values were found, ranging from 99.8% for length to 87.6% for depth. For the pollen grain characters measured, the repeatability values ranged from 67.6% for percent fertility to 23.1% for polar diameter. For the pistil characters measured, the repeatability values ranged from 94.0% for style width to 49.6% for total pistil length. These results indicate that genotype and environment influence anther, pollen grain and pistil characters. Variation in some of these morphological aspects could influence the consistency and interpretation of male transmission studies on both the applied and evolutionary levels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050036
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  • 5
    Electronic Resource
    Electronic Resource
    Genetic and environmental variation in anther, pollen and pistil dimensions in sesame (1996)
    Springer
    Sexual plant reproduction 9 (1996), S. 228-232 
    Details
    ISSN: 1432-2145
    Keywords: Male transmission ; Anther dimensions ; Pollen dimensions ; Pistil dimensions ; Sesame ; Sesamum indicum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Measurements of anther (length, width, depth), pollen grain (percent fertility, polar diameter, equatorial diameter, polar diameter/equatorial diameter ratio, volume) and pistil (stigma length, style length, ovary length, total pistil length, stigma width, style width, ovary width) were taken on 12 diverse sesame (Sesamum indicum L.) genotypes on each of four collection dates in 1994. Highly significant differences among genotype means were obtained for all characters except polar diameter. Highly significant differences among environment (collection date) means were found for ten of the 15 characters measured. Highly significant genotype x environment interactions were obtained for all characters except anther length. For the anther characters measured, relatively high repeatability values were found, ranging from 99.8% for length to 87.6% for depth. For the pollen grain characters measured, the repeatability values ranged from 67.6% for percent fertility to 23.1% for polar diameter. For the pistil characters measured, the repeatability values ranged from 94.0% for style width to 49.6% for total pistil length. These results indicate that genotype and environment influence anther, pollen grain and pistil characters. Variation in some of these morphological aspects could influence the consistency and interpretation of male transmission studies on both the applied and evolutionary levels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02173103
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