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  • 1
    ISSN: 1435-1536
    Keywords: Wool ; nonkeratinous proteins ; enzymatic hydrolysis ; pronaseE ; extent of digestion ; (α + ε)-aminogroups ; microbial investigations ; fractionating of the keratoses ; internal volume ; mercury porosimetry ; centrifugal swelling test
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The nonkeratinous proteins of wool can be partly or completely dissolved by controlled enzymatic digestion. A comparison between the keratose content of intact wool and that of wool which has been hydrolysed for 96 h with pronase confirms that specifically all the nonkeratinous components are dissolved out of the wool composite by pronase. The influence of microbial infection can be excluded. The residue of wool after the hydrolysis, i. e., a wool low in nonkeratinous proteins, has a higher internal volume, as measured by mercury porosimetry. The centrifugal swelling test shows that these wool samples also have increased hygroscopy.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Colloid & polymer science 264 (1986), S. 171-175 
    ISSN: 1435-1536
    Keywords: Wool ; lipid isolation ; cell membrane complex ; phospholipids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The internal lipids of wool were isolated after solubilizing the wool keratin with a mixture of papain and dithioerythritol 1.4. Thin layer chromatography of the internal lipids was applied to classify the extracted components. The internal wool lipids gave 11 spots of which some were identified as cholesterol and free fatty acids, C-16 and C-18 being predominant. A very small amount of triglycerides and cholesterol esters were also found. A characteristic difference between internal wool lipids and wool wax appears to be the limited number of well defined components of which free cholesterol and fatty acids constitute the main bulk. Furthermore another feature of keratin membrane lipids is the extremely reduced phospholipid: cholesterol ratio of 0.3. The internal lipids originate mainly from the cell membrane complex of the fiber. The existence of a chemically modified bilayer membrane structure without the essential phospholipids must be taken into consideration.
    Type of Medium: Electronic Resource
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