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cDNA cloning of β-tubulin gene and organization of tubulin genes in Vigna radiata (mung bean) genome (1987)

Raha, Debasish ; Sen, Kunal ; Biswas, B. B.

Springer

Plant molecular biology 9 (1987), S. 565-571

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ISSN: 1573-5028

Keywords: Vigna radiata ; tubulin ; cDNA cloning ; mapping ; genomic organization

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract We isolated an almost full-length cDNA clone containing β-tubulin gene from a partial cDNA library of mung bean using chicken cDNA as probe. Cross-hybridization with chicken β-tubulin cDNA and positive hybridization-selection and translation of mung bean mRNA established that this clone contains β-tubulin sequences. We studied the organization of tubulin genes in mung bean. In this plant tubulin genes are organized in tandem repeats of alternating α- and β-tubulin genes. The 5.6 kb basis repeat unit which contains both α- and β-tubulin genes is repeated twenty times per haploid genome.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00020533

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