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  • 1
    Electronic Resource
    Electronic Resource
    Structural constraints in expansion segments from a midge 26S rDNA (1995)
    Springer
    Journal of molecular evolution 41 (1995), S. 1016-1021 
    Details
    ISSN: 1432-1432
    Keywords: Chironomus ; LSU rRNA evolution ; Expansion segments ; Compensatory mutations ; rRNA processing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract DNA sequences representing approximately 40% of the large-subunit rRNA gene from the lower dipteran Chironomus thummi were analyzed. Once aligned with their Drosophila counterparts, sequence and base content comparisons were carried out. Sequence identity was found to be high overall, except for six regions that displayed a local bias in nucleotide composition toward AT. These regions were identified as expansion segments D3, D4, D5, D6, D7a, and D12. Besides base sequence divergence, differences in length were observed between the respective variable domains of the two species, particularly for D7a. Prediction of secondary structure showed that the folding of the Chironomus expansion segments analyzed is in agreement with the general patterns proposed for eukaryotic LSU rRNA. The comparison with Drosophila revealed also that the Chironomus secondary structures of the variable domains are supported by multiple compensatory substitutions or even compensatory insertions. Chironomus D7a displayed an unusual structural feature with respect to the insect D7a models that have been inferred up to now. The structural constraint observed in the expansion segments of Diptera so distantly related as midges and Drosophila suggests that these regions contribute to some functional role. Concerning the D7a of insects so far analyzed, there can be, in addition to a conserved secondary structure, a nucleotide composition constraint that might be important for the process giving rise to the alpha and beta halves of the 26S rRNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00173183
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of two new members of the l-aminocyclopropane-l-carboxylate synthase-encoding multigene family in mung bean
    Amsterdam : Elsevier
    Gene 123 (1993), S. 249-253 
    Details
    ISSN: 0378-1119
    Keywords: Vigna radiata ; polymerase chain reaction ; recombinant DNA ; thylene
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0378-1119(93)90132-M
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  • 3
    Electronic Resource
    Electronic Resource
    The Balbiani ring 6 induction in Chironomus
    Amsterdam : Elsevier
    Biology of the Cell 71 (1991), S. 11-16 
    Details
    ISSN: 0248-4900
    Keywords: BR genes ; Chironomus ; polytene chromosomes ; regulation of BRs ; sp-I secretory proteins ; sugar effect on BRs
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0248-4900(91)90046-P
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  • 4
    Electronic Resource
    Electronic Resource
    Differential expression of two calmodulin genes in response to physical and chemical stimuli (1994)
    Springer
    Plant molecular biology 24 (1994), S. 757-766 
    Details
    ISSN: 1573-5028
    Keywords: touch ; calcium ; indole-3-acetic acid ; salt stress ; light ; signal transduction ; Vigna radiata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two different calmodulin (CaM) cDNAs (MBCaM-1 and MBCaM-2) were isolated from a vigna radiata λgt 11 library by screening with a heterologous Arabidopsis cDNA probe (TCH-1). Both cDNAs are 85% homologous inside the coding region but are highly divergent outside this region. The polypeptides encoded by MBCaM-1 and MBCaM-2 are identical except for two conservative substitutions at positions 7 and 10. Southern analysis revealed that both cDNAs are encoded by different genes. Expression studies revealed different patterns of expression of both genes. MBCaM-1 mRNA exhibited a dramatic transient increase in response to touch, while MBCaM-2 expression showed a steady but small increase as compared to MBCaM-1. When plants were grown in complete darkness MBCaM-1 was undetectable and MBCaM-2 exhibited very low levels of expression. One hour after exposure of etiolated seedlings to light MBCaM-1 showed no change, while MBCaM-2 expression was increased. After a 6 h exposure to light there was an induction of both MBCaM-1 and MBCaM-2; however, the magnitude of this increase was much greater for MBCaM-2. When plants were grown under a 16 h light/8 h dark cycle the mRNA levels for MBCaM-1 were lower during the light period and increased during the beginning of the night cycle, while MBCaM-2 showed no change. Plants treated with indole-3-acetic acid had a peak in MBCaM-1 expression 6 h after treatment initiation with a slight decline 3 h after the peak, while MBCaM-2 showed a steady but small increase over time as compared to MBCaM-1. When plants were subjected to salt stress they showed an increase in MBCaM-1 expression 2 h after treatment initiation reaching a maximum after 4 h with no further increase after 6 h, while MBCaM-2 remained unchanged over the time course.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00029857
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  • 5
    Electronic Resource
    Electronic Resource
    Identification and characterization of a full-length cDNA encoding for an auxin-induced 1-aminocyclopropane-1-carboxylate synthase from etiolated mung bean hypocotyl segments and expression of its mRNA in response to indole-3-acetic acid (1992)
    Springer
    Plant molecular biology 20 (1992), S. 425-436 
    Details
    ISSN: 1573-5028
    Keywords: 1-aminocyclopropane-1-carboxylic acid (ACC) ; ACC synthase ; indole-3-acetic acid (IAA) ; ethylene ; Vigna radiata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 1-Aminocyclopropane-1-carboxylate (ACC) synthase (EC 4.4.1.14) is the key regulatory enzyme in the ethylene biosynthetic pathway. The identification and characterization of a full-length cDNA (pAIM-1) 1941 bp in length for indole-3-acetic acid (IAA)-induced ACC synthase is described in this paper. The pAIM-1 clone has an 87 bp leader and a 402 bp trailing sequence. The open reading frame is 1452 bp long encoding for a 54.6 kDa polypeptide (484 amino acids) which has a calculated isoelectric point of 6.0. In vitro transcription and translation experiments support the calculated molecular weight and show that the enzyme does not undergo processing. Eleven of the twelve amino acid residues which are conserved in aminotransferases are found in pAIM-1. The sequence for pMAC-1 which is one of the 5 genes we have identified in mung bean is contained in pAIM-1. pAIM-1 shares between 52 to 65% homology with previously reported sequences for ACC synthase at the protein level. There is little detectable pAIM-1 message found in untreated mung bean tissues; however, expression is apparent within 30 min following the addition of 10 μM IAA reaching a peak after approximately 5 h with a slight decrease in message after 12 h. These changes in message correlate with changes in ACC levels found in these tissues following treatment with 10 μM IAA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040602
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  • 6
    Electronic Resource
    Electronic Resource
    Calcium-dependent protein kinase gene expression in response to physical and chemical stimuli in mungbean (Vigna radiata) (1996)
    Springer
    Plant molecular biology 30 (1996), S. 1129-1137 
    Details
    ISSN: 1573-5028
    Keywords: calcium ; cycloheximide ; indole-3-acetic acid (IAA) ; mechanical strain ; protein kinase ; salt stress ; touch ; Vigna radiata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Protein kinases are important in eukaryotic signal transduction pathways. In this study we designed degenerate oligonucleotides corresponding to two conserved regions of protein kinases and using the polymerase chain reaction (PCR) have amplified a 141 bp fragment of DNA from mungbeans (Vigna radiata Rwilcz cv. Berken). Sequence analysis of the PCR products indicates that they encode several putative protein kinases with respect to their identity with other known plant protein kinases. Using one of the six fragments (CPK3-8), we isolated a 2022 bp cDNA (VrCDPK-1) from a Vigna radiata λgt11 library. VrCDPK-1 has a 96 bp 5′-untranslated region and a 465 bp 3′-untranslated region and an open reading frame of 1461 bp. VrCDPK-1 contains all of the conserved regions commonly found in calcium dependent protein kinases (CDPK). VrCDPK-1 shares 24 to 89% sequence identity with previously reported sequences for plant CDPKs at the protein level. southern analysis revealed the presence of several copies of the CDPK gene. VrCDPK-1 expression was stimulated when mungbean cuttings were treated with CaCl2, while treatment with MgCl2 had no effect. We are reporting for the first time a CDPK gene in mungbean which is inducible by mechanical strain. Cuttings treated with indole-3-acetic acid (IAA) or subjected to salt stress showed an increase in VrCDPK-1 expression. There was a dramatic stimulation in VrCDPK-1 expression 6 h after cuttings were treated with cycloheximide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019547
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  • 7
    Electronic Resource
    Electronic Resource
    Identification and characterization of three putative genes for 1-aminocyclopropane-1-carboxylate synthase from etiolated mung bean hypocotyl segments (1992)
    Springer
    Plant molecular biology 18 (1992), S. 793-797 
    Details
    ISSN: 1573-5028
    Keywords: 1-aminocyclopropane-1-carboxylic acid (ACC) ; ACC synthase ; S-adenosylmethionine (AdoMet) ; ethylene ; polymerase chain reaction (PCR) ; Vigna radiata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The polymerase chain reaction (PCR) was used to produce 3 putative clones for ACC synthase from etiolated mung bean (Vigna radiata Rwilcz cv. Berken) hypocotyls. This was accomplished by utilizing genomic DNA from mung bean and degenerate primers made from information derived from highly conserved regions of ACC synthase from different plant tissues. The total length of pMAC-1, pMAC-2 and pMAC-3 are 308, 321, and 326 bp, respectively, all of which code for 68 amino acids. The introns for pMAC-1, pMAC-2 and pMAC-3 are 92, 105, and 110 bp, respectively. The degrees of homology at the DNA level for each of these clones is ca. 80% in their coding region and ca. 50% in their respective introns. This is the first report providing evidence that there are at least 3 genes for ACC synthase in etiolated mung bean.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020022
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  • 8
    Electronic Resource
    Electronic Resource
    The c-terminal DNA-binding domain ofChironomus BR gene products shows preferential affinity for (dA,dT)-rich sequences (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 422-427 
    Details
    ISSN: 1617-4623
    Keywords: Chironomus ; Secretory proteins ; DNA-binding ; A.T-rich DNA tracts ; DNA bending
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Balbiani ring genes (BRs), the most active loci in the polytene chromosomes of the salivary gland of the midgeChironomus (Diptera), code for secretory giant peptides (the sp-I family). Evidence previously reported indicated that the conserved C-terminal region of proteins of the sp-I family had DNA-binding properties (assayed with sp-Ia), and one such region, derived fromBR2.2, which codes for the product sp-Ib, might occur as a stable independent peptide, being transferred to the nucleus where it is detectable in the largeBRs (BR1 andBR2), among other structures, by immunostaining. Here, we show that the C-terminal portion of one of theBR gene products, expressed as a glutathione-S-transferase fusion protein shows preferential affinity for A.T-rich sequences and binds with varying affinity to restriction fragments of the A.T-rich BR1 promoter. The binding was inhibited by distamycin, suggesting that the interaction involves the minor groove of the DNA. Analysis of the promoter fragments by gel electrophoresis indicated that most appeared to present a conspicuous bend, as deduced from their anomalous electrophoretic mobilities. Furthermore, the affinity of the C-terminal domain for the different promoter fragments appeared to correlate with the degree of bending. Thus, the C-terminal domain might play a role in controlling gene expression by binding to A.T-rich sequences, including those of theBR genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02172370
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