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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 167 (1976), S. 387-405 
    ISSN: 1432-0878
    Keywords: Rabbit pars intermedia ; Ultrastructure ; Developing tissue ; Cytodifferentiation ; Organ culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pars intermedia (PI) tissue from fetal, perinatal, neonatal and juvenile rabbits has been maintained in organ culture for up to nine weeks after explantation. Autoradiography showed that DNA synthesis took place for at least 22 days of culturing. PI-glandular cells and interstitial cells remain identifiable throughout this period but ACT-type cells were recognised only up to six weeks. Material from fetal and perinatal animals had a higher proportion of surviving cells than that from adult animals. The degree of differentiation achieved by PI-glandular cells in vitro appears to depend on three factors: i) the stage of development reached before explantation; ii) the original topographic position in the PI tissue before explantation; and iii) the position in the explant in relation to the gas-liquid interphase.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 169 (1976), S. 485-500 
    ISSN: 1432-0878
    Keywords: Pars intermedia, rabbit ; Adult, young adult tissue ; Cytodifferentiation ; Organ culture ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fully differentiated rabbit pars intermedia has been maintained successfully for over six weeks as organ culture. PI-glandular cells are the commonest type throughout the culture. They retain their differentiated condition for at least five weeks. Autoradiography indicates that cell division occurs in cultures for at least two weeks. Dense granules are seen in the intercellular, perivascular and vascular spaces and are taken to indicate secretory activity under the conditions of culture. Some interstitial cells remain recognisable throughout the culture and ACT-type cells for more than two weeks. Other interstitial cells lose their usual appearance and become peripheral dark cells. Modification of cytoplasmic organelles, including distortion and darkening of mitochondria, increase in intracellular filaments and enlargement and liberation of the content of the PI-vesicles are reported.
    Type of Medium: Electronic Resource
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