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  • Engineering  (17)
  • Two-dimensional polyacrylamide gel electrophoresis  (8)
  • Wiley-Blackwell  (25)
  • 1
    Electronic Resource
    Electronic Resource
    Preface (1995)
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 21 (1995), S. iii 
    Details
    ISSN: 0271-2091
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/fld.1650211002
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  • 2
    Electronic Resource
    Electronic Resource
    '98 Escherichia coli SWISS-2DPAGE database update (1998)
    Weinheim : Wiley-Blackwell
    Electrophoresis 19 (1998), S. 1960-1971 
    Details
    ISSN: 0173-0835
    Keywords: Two-dimensional polyacrylamide gel electrophoresis ; Escherichia coli ; SWISS-2DPAGE database ; Immobilized pH gradient ; Sequence tag ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: The combination of two-dimensional polyacrylamide gel electrophoresis (2-DPAGE), computer image analysis and several protein identification techniques allowed the Escherichia coli SWISS-2DPAGE database to be established. This is part of the ExPASy molecular biology server accessible through the WWW at the URL address http://www.expasy.ch/ch2d/ch2d-top.html. Here we report recent progress in the development of the E. coli SWISS-2DPAGE database. Proteins were separated with immobilized pH gradients in the first dimension and sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the second dimension. To increase the resolution of the separation and thus the number of identified proteins, a variety of wide and narrow range immobilized pH gradients were used in the first dimension. Micropreparative gels were electroblotted onto polyvinylidene difluoride membranes and spots were visualized by amido black staining. Protein identification techniques such as amino acid composition analysis, gel comparison and microsequencing were used, as well as a recently described Edman “sequence tag” approach. Some of the above identification techniques were coupled with database searching tools. Currently 231 polypeptides are identified on the E. coli SWISS-2DPAGE map: 64 have been identified by N-terminal microsequencing, 39 by amino acid composition, and 82 by sequence tag. Of 153 proteins putatively identified by gel comparison, 65 have been confirmed. Many proteins have been identified using more than one technique. Faster progress in the E. coli proteome project will now be possible with advances in biochemical methodology and with the completion of the entire E. coli genome.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150191114
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  • 3
    Electronic Resource
    Electronic Resource
    Human blood platelet protein map established by two-dimensional polyacrylamide gel electrophoresis (1995)
    Weinheim : Wiley-Blackwell
    Electrophoresis 16 (1995), S. 1152-1159 
    Details
    ISSN: 0173-0835
    Keywords: Two-dimensional polyacrylamide gel electrophoresis ; Human blood platelets ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Two-dimensional (2-D) maps of cytosol and enriched-membrane platelet proteins has allowed the identification of more than 25 spots by three different methods: matching of the platelet gels with other 2-D reference maps, immunoblotting with chemiluminescence detection, and N-terminal sequencing. Different G protein (guanosine triphosphate-binding protein) subunits, cytoskeletal proteins, and proteins common to the human liver, red blood cells and plasma were identified. The two platelet protein maps presented here contribute to the project of identification of human cell and body fluid proteins. They may serve as working tools since platelets are popular models for the study of central nervous system neurotransmitter systems and stimulus-response coupling mechanisms.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.11501601191
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  • 4
    Electronic Resource
    Electronic Resource
    Tensile stresses around boreholes due to transient fluid flow (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    International Journal for Numerical and Analytical Methods in Geomechanics 17 (1993), S. 659-667 
    Details
    ISSN: 0363-9061
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Geosciences
    Notes: We present solutions for the effective stress induced by gas flow through a porous solid into a borehole resulting from sudden pressure reduction. Tensile effective stress that exceeds the strength of the solid will lead to borehole failure. This has applications to the intentional creation of cavities, relevant to the efficient recovery of coalbed methane, and the avoidance of borehole stability problems in conventional gas production.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nag.1610170905
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  • 5
    Electronic Resource
    Electronic Resource
    Recent progress in the development and understanding of SUPG methods with special reference to the compressible Euler and Navier-Stokes equationsBased on an invited lecture.Supported by NASA Langley Research Center under Grant NASA-NAG-1-361 and the IBM Almaden Research Center under Grant No. 604912. (1987)
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 7 (1987), S. 1261-1275 
    Details
    ISSN: 0271-2091
    Keywords: Advection-diffusion equation ; Advective-diffusive systems ; Artificial-diffusion ; Compressible flows ; Discontinuous Galerkin method ; Entropy Error analysis ; Euler equations ; Finite elements ; Galerkin method ; Hyperbolic systems ; Incompressible flow ; Navier-Stokes equations ; Petrov-Galerkin method ; Space-time formulation ; Upwind methods ; Weighted residual methods ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: SUPG methods were originally developed for the scalar advection-diffusion equation and the incompressible Navier-Stokes equations. In the last few years successful extensions have been made to symmetric advective-diffusive systems and, in particular, the compressible Euler and Navier-Stokes equations. New procedures have been introduced to improve resolution of discontinuities and thin layers. In this paper a brief overview is presented of recent progress in the development and understanding of SUPG methods.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/fld.1650071108
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  • 6
    Electronic Resource
    Electronic Resource
    A case study in parallel computation: Viscous flow around an ONERA M6 wing (1995)
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 21 (1995), S. 877-884 
    Details
    ISSN: 0271-2091
    Keywords: ONERA M6 wing ; parallel computing ; viscous flow ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: We examine the solution of a practical engineering problem on a parallel computer. The problem involves the steady laminar viscous flow about an ONERA M6 wing and the computer is a 64-processing-node Connection Machine CM-5E. We show that efficient domain decomposition procedures lead to a balanced load on the processors and low communication times. The net result is that solutions can be attained in roughly 20 min elapsed time for a 48,011-node, 266,566-element unstructured mesh. We conclude that this is sufficiently fast to support the design process.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/fld.1650211008
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  • 7
    Electronic Resource
    Electronic Resource
    Reduction scheme for some structural eigenvalue problems by a variational theorem (1976)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 10 (1976), S. 845-852 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: An accurate reduction scheme for structural eigenvalue problems is deduced from a variational theorem in which the displacement, velocity and/or momentum fields are taken to be independent.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620100411
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  • 8
    Electronic Resource
    Electronic Resource
    A simple and efficient finite element for plate bending (1977)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 11 (1977), S. 1529-1543 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A simple and efficient finite element is introduced for plate bending applications. Bilinear displacement and rotation functions are employed in conjunction with selective reduced integration. Numerical examples indicate that, despite its simplicity, the element is surprisingly accurate.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620111005
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  • 9
    Electronic Resource
    Electronic Resource
    Generalization of selective integration procedures to anisotropic and nonlinear media (1980)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 15 (1980), S. 1413-1418 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A new finite element procedure is introduced for the analysis of nearly-incompressible media. The approach may be simply implemented by a small change of the standard technique, and is applicable to arbitrary anisotropic and/or nonlinear media. The procedure is shown to specialize to the selective integration and mean-dilatation formulations under appropriate hypothesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620150914
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  • 10
    Electronic Resource
    Electronic Resource
    Analysis of some fully-discrete algorithms for the one-dimensional heat equation (1985)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 21 (1985), S. 163-168 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A fully discrete stability and accuracy analysis of some algorithms for the one-dimensional heat equation is presented. Results illustrate that 2-pass explicit schemes which simultaneously employ lumped and coupled capacity matrices are capable of improved performance over the standard 1-pass explicit scheme.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620210113
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