ISSN:
1432-072X
Keywords:
Formylmethanofuran dehydrogenase
;
Tungsten enzymes
;
Molybdopterin dinucleotides
;
Methanogenesis
;
Archaea
;
Archaebacteria
;
Methanobacterium thermoautotrophicum
;
Methanobacterium wolfei
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Methanobacterium thermoautotrophicum (strain Marburg) was found to grow on media supplemented with tungstate rather than with molybdate. The Archaeon then synthesized a tungsten iron-sulfur isoenzyme of formylmethanofuran dehydrogenase. The isoenzyme was purified to apparent homogeneity and shown to be composed of four different subunits of apparent molecular masses 65 kDa, 53 kDa, 31 kDa, and 15 kDa and to contain per mol 0.4 mol tungsten, 〈0.05 mol molybdenum, 8 mol non-heme iron, 8 mol acid-labile sulfur and molybdopterin guanine dinucleotide. Its molecular and catalytic properties were significantly different from those of the molybdenum isoenzyme characterized previously. The two isoenzymes also differed in their metal specificity: the active molybdenum isoenzyme was only synthesized when molybdenum was available during growth whereas the active tungsten isoenzyme was also generated during growth of the cells on molybdate medium. Under the latter conditions the tungsten isoenzyme was synthesized containing molybdenum rather than tungsten.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00248696
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