ISSN:
1432-2242
Keywords:
Anther culture
;
Pollen callus (plantlet)
;
Induction frequency
;
Triticum aestivum
;
Agropyron glaucum
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Experimental results showed that the use of potato extract as a basic component of culture medium had a promoting effect on producing calli in anther culture of the intergeneric hybrids of Triticum aestivum × Triticum-Agropyron (intermediate type). The induction frequencies of pollen callus on the Potato-II medium containing potato extract as the main component was much higher than that found on N6 and W5 media. The induction frequencies of pollen callus and green plantlets in four intergeneric hybrid material inoculated at the late-uninucleate pollen stage were all higher than those inoculated at the mid-uninucleate stage. Appropriate increases in culture temperature significantly increased pollen callus induction frequencies of the intergeneric hybrids. The genotype and physiological state of anther donor plants also influenced pollen callus and green plantlet induction frequencies.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00304914
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