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  • 1
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; T. durum ; Aegilops longissimum ; Dasypyrum villosum ; Endonuclease ; Cytoplasm donor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To elucidate the phylogenetic relationships and cytoplasmic types, restriction endonuclease fragment patterns of chloroplast (cp) and mitochondrial (mt) DNAs isolated from two different accessions of Dasypyrum villosum (L.) candargy were compared with those of tetraploid wheat (Triticum durum Desf., PI265007), hexaploid wheat (Triticum aestivum L., cv Chinese Spring), Aegilops longissimum (S. and M., in Muschli) Bowden and Hordeum vulgare L. T. aestivum and T. durum had identical restriction patterns for their cp and mtDNAs in digestions with four different enzymes. Likewise, no differences were found between the restriction fragment patterns of two accessions of D. villosum. But, there were distinct differences in chloroplast and mitochondrial DNA restriction fragment patterns between D. villosum and tetraploid and hexaploid wheats. A. longissimum (G609) showed a similar pattern to those wheats for PstI digestion of cpDNA. Organellar DNA from Hordeum vulgare (cv Himalaya) showed a distinctly different restriction pattern from those of wheat and D. villosum. These results suggest that D. villosum is unlikely to be the donor of cytoplasm to wheats, and its cytoplasmic organelles were also different from those of A. longissimum.
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  • 2
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; T. timopheevii ; Wheat ; Photoperiod ; Vernalization ; Male sterility ; Alloplasmic hybrids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Studies were conducted to determine the influence of the male sterility-inducing cytoplasm of Triticum timopheevii (Zhuk.) Zhuk. on response of several common winter wheat (T. aestivum L.) nuclear genotypes to photoperiod and vernalization. Comparative studies of cytoplasmic substitution lines provide information on the role of the cytoplasmic genetic mechanism in growth and development. In the case of cytoplasmic male sterility-based hybrid production systems, ubiquity of sterility-inducing cytoplasm in derived hybrids warrants thorough characterization of its influence on plant phenotype. Factorial combinations of cytoplasm (T. timopheevii and T. aestivum), nuclear genotype, and photoperiod or vernalization treatments were evaluated under hydroponic conditions in controlled environment chambers. Interaction of cytoplasm, photoperiod, and nuclear genotype was significant in one or more experiments for days to anthesis and potential spikelet number, and interaction of cytoplasm, vernalization, and nuclear genotype was significant for days to spike emergence. Long day length was associated with increased percentage seed set in one study, but interactions of photoperiod and cytoplasm were not detected for percentage seed set. Interactions involving cytoplasm and photoperiod or vernalization were interpreted as evidence of the existence of genetic factors in cytoplsam of T. timopheevii which alter photoperiod or vernalization responses of alloplasmic plants relative to responses exhibited by euplasmic plants. Since photoperiod and vernalization responses are critical to adaptation, T. timopheevii cytoplasm can alter adaptability of T. aestivum. The specific effect would be nuclear genotype dependent, and does not appear to be of a magnitude greater than that induced by nuclear genetic variability at loci conditioning photoperiod or vernalization responses or other adaptation-determining characteristics. Normal multilocation/year testing of alloplasmic hybrids should therefore adequately identify zones of adaptation.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 65 (1983), S. 41-46 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Wheat ; Protein ; Mutation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Poor adaptability or functional quality of much germplasm used for breeding high-protein hard red winter wheats prompted mutagenesis as an alternative means of increasing grain protein content. Four hard red winter wheat genotypes — KS644 (‘Triumph// Concho/Triumph’), ‘Kaw’, ‘Parker’, and ‘Shawnee’ — were treated with 0.40 M ethyl methanesulfonate (EMS). Advanced lines (M8-M10) were selected that had a 3-year mean grain protein advantage of 0.7% to 2.0% over controls. Increased grain protein content was generally associated with decreased grain yield and kernel weight, but some high-protein mutant lines had yields or kernel weights similar to those of original genotypes. Changes in height and lodging induced by EMS were generally favorable, most mutants being shorter and lodging less than controls, but blooming date was generally delayed, a deleterious change. One line also changed from resistant to segregating for wheat soil-borne mosaic virus. Mutant lines might be utilized in cross-breeding programs, particularly if negative pleiotropic effects and linkages are absent.
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  • 4
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; preharvest sprouting ; dormancy ; α-amylase ; falling number ; inhibitors ; embryo response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Resistance to preharvest sprouting has been observed in several white wheat (Triticum aestivum L.) genotypes, but the mode of resistance has not been determined. Studies were conducted to characterize the strong preharvest sprouting resistance in Clark's Cream genotype to facilitate breeding for the trait. Clark's Cream and five other hard red and white wheat genotypes were grown in the glasshouse and field and effects of simulated rain, embryo maturity, inhibitors in floral parts and GA, endogenous inhibitors, and ABA and GA combinations on sprouting were measured. The six genotypes differed significantly in sprouting after simulated rain, α-amylase activity, and response to GA and ABA. Embryo maturity and inhibition levels in floral parts and caryopses were nearly similar for all genotypes. Clark's Cream appeared to differ most in high embryo sensitivity, which was fairly nonspecific for ABA, GA, and endogenous inhibitor, and in low α-amylase production during sprouting. Breeding procedures that lead to the preharvest sprouting resistance of Clark's Cream are discussed.
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  • 5
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; breeding ; preharvest sprouting ; heritability ; variation ; α-amylase ; falling number
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Preharvest sprouting resistance is a major breeding criterion in many regions where white wheat (Triticum aestivum L.) is produced. Availability of genetic sources of sprouting resistance should significantly advance this goal. Objectives of the present investigation were to determine heritabilities and genetic variation of sprouting resistance in progeny of six susceptible genotypes and Clark's Cream, a cultivar with excellent sprouting resistance. Sixty-five randomly selected F2:F3 lines from the six parental combinations were evaluated in blocks-within-replication designs at two locations during 1984–85. Falling number, α-amylase activity, and sprouting percentage in simulated rain were usually more favorable in F2:F4 grain than in grain of the susceptible parents and frequently equalled levels in grain of the resistant parent. Broad sense heritability estimates were moderate to high for falling number and α-amylase activity, low to moderate for visual sprouting, and inconsistent for embryo germination in ABA. Phenotypic correlations with sprouting were significant most often for falling number and least often for the agronomic traits, days to heading and kernel weight. We concluded that increased preharvest sprouting resistance is an attainable objective when genetic sources of strong resistance and appropriate selection criteria, such as sprouting after simulated rain, are used.
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  • 6
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; breeding ; grain yield ; grain quality ; preharvest sprouting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Hard white winter wheat (HWWW) occupies a very limited area of the USA, but its purported advantages suggest that its production in the major hard red winter wheat (HRWW) region may be feasible. Objectives of our investigations were to develop experimental HWWW lines that combined desirable attributes-grain yield, functional grain quality, and resistance to preharvest sprouting-in single genotypes for comparison with popular cultivars in the major US RHWW region. Forty-four lines from seven parental combinations were tested in randomized complete block designs at three Kansas locations during the 1981–82 and 1982–83 seasons. Agronomic traits, grain yield, grain quality, and preharvest sprouting were measured. Plant characteristics and grain yield were similar in the HWWW experimental lines and the HRWW check cultivar, Newton. Mean grain SDS-sedimentation value and grain protein content of most experimental lines equaled or exceeded that of the check. Dough mixing times frequently were shorter for the experimental lines than for the check cultivar, whereas loaf volumes were greater. Falling number usually was similar in all geneotypes, but α-amylase was higher in field-harvested grain of white lines than the check; both measures were more favorable than grain trade standards. We concluded that production of high yielding, high quality hard white winter wheat genotypes is feasible in the US ‘breakbasket’.
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  • 7
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; pre-harvest sprouting ; germination ; α-amylase enzyme ; gibberellic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Sensitivity to GA in non-Gai genome winter wheat (Triticum aestivum L.) cultivars was investigated to determine magnitude of variation of the trait, its association with other traits, and effects of geographical location of production. α-Amylase enzyme activity was measured before and after treatment with gibberellic acid in 18 cultivars grown at one location and in five cultivars grown at six locations. Dye-labeled starch and agar-starch media procedures were used for the first and second experiments, respectively. α-Amylase activity differed significantly among the 18 cultivars after germination in water, germination in GA, and in absolute and relative sensitivity to GA. Cultivars that reacted similarly to GA had some common ancestors in their pedigrees, and α-amylase activity and sensitivity to GA were significantly negatively associated with seed weight. α-Amylase activity differed between tall and semi-dwarf cultivars in the second experiment, but not in the first experiment. The magnitude of variation in α-amylase activity and its relationship to preharvest sprouting susceptibility of the cultivars suggested that the trait can be modified to improve seed dormancy. Significant interactions between cultivar responses to GA and geographical location of grain production suggested that selection should be carried out in several environments.
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  • 8
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; protein content ; grain yield ; mixing time ; soil-borne mosaic virus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The wheat (Triticum aestivum L.) cultivar Lancota has genetic potential to produce grain with higher protein content than most other cultivars grown in the hard winter wheat region. It has not consistently expressed full potential for grain protein content outside its area of development. Experiments were conducted to determine genetic variability for grain protein content in Lancota and to utilize that variability to select genotypes with high grain protein content. Approximately 1600 lines were screened to 37 high-protein selections that varied in yield, test weight, flour mixing time, blooming date, height, and reaction to wheat soil-borne mosaic virus (WSBM). Nine promising selections (KS80476, KS80478, KS80480, KS80488, KS80490, KS80491, KS80497, KS80499, and KS80500) had grain protein advantage over Lancota of 0.5 to 1.0% and equalled or exceeded Lancota in yield or test weight. Those selections were resistant to WSBM and satisfactory or better in mixing properties than Lancota. The highest protein selection (KS80496) had a mean protein advantage of 1.5% over Lancota but exhibited a short mixing time of 1 7/8 min. The absence of correlation between some years indicated strong environmental influence on protein content. We concluded that adequate genetic variability existed in the high-grain protein cultivar Lancota to select lines that express the high protein potential better than the original cultivar outside its area of development.
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  • 9
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; kernel color ; protein content ; inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Utilization of high-protein hard red wheat germplasm in breeding high-protein hard white winter wheats for the U.S. Great Plains raised concern regarding possible genetic relationships between kernel color and protein content. Segregating F3 and F4 populations from reciprocal crosses and backcrosses involving high-protein hard red winter wheat cultivar Plainsman V and normal-protein hard white winter wheat line KS75216 were examined. Nonsignificant regression and correlation coefficients in the F3 generations of KS75216/Plainsman V, KS75216//KS75216/Plainsman V and Plainsman V//KS75216/Plainsman V indicated the absence of genetic relationships between kernel color and protein content. Therefore, despite the presence of genes for protein content and kernel color on the same chromosomes (3A, 3B and 3D), kernel color and protein content appeared as independent traits. A small but significant negative relationship between white kernel color and high protein in Plainsman V/KS75216 was attributable to the possible presence of alien genetic material in the parentage of Plainsman V. Chi-square tests indicated that Plainsman V is a mixture of genotypes for kernel color; most genotypes carry two dominant genes for red color and a few carry one or three. Genetic control of grain protein appeared to be complex. Partial dominance for high protein was indicated in the F3 generation but a generally continuous distribution and transgressive segregation also suggested other genes functioned additively. Heritability estimates by parent-offspring (F3-F4) regression were sufficiently high to ensure genetic progress in the selection of high-protein lines in the red x white wheat crosses. We concluded that development of high-protein cultivars is as feasible for white wheats as for red wheats.
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