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  • Trifolium repens  (4)
  • Springer  (4)
  • Oxford University Press
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  • Springer  (4)
  • Oxford University Press
  • Elsevier  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Biochemical characterisation of the Li locus, which controls the activity of the cyanogenic β-glucosidase in Trifolium repens L. (1982)
    Springer
    Plant molecular biology 1 (1982), S. 169-181 
    Details
    ISSN: 1573-5028
    Keywords: cyanogenesis ; β-glucosidase ; linamarase ; Trifolium repens ; null loci
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The cyanogenic β-glucosidase (linamarase) was purified from white clover leaf tissue. The enzyme is a homodimer with a molecular weight of 105 300–103 400 daltons estimated from molecular exclusion chromatography. The effect of buffer ions on the pH optimum and charge properties of the enzyme are presented. A combination of molecular exclusion chromatography and CM cellulose ion exchange chromatography purified linamarase 16 fold to a single 62 000 dalton polypeptide on SDS polyacrylamide gel electrophoresis. This polypeptide represented about 5% of the total soluble leaf protein and can be seen as a prominent band in SDS polyacrylamide gel electrophoresis of crude leaf extracts from Li Li plants. Screening backcross progeny showed that extracts from li li plants, which have no linamarase activity, lack this 62 000 dalton polypeptide. Linamarase is the major glycoprotein in white clover leaf extracts which binds to Concanavalin A-Sepharose.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021030
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  • 2
    Electronic Resource
    Electronic Resource
    Restriction fragment length polymorphism segregation analysis of the Li locus in Trifolium repens L. (1990)
    Springer
    Plant molecular biology 14 (1990), S. 407-414 
    Details
    ISSN: 1573-5028
    Keywords: cDNA ; cyanogenesis ; β-glucosidase ; RFLP ; Trifolium repens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Li locus in white clover controls the presence of cyanogenic β-glucosidase (linamarase) activity in leaf tissue, such that plants homozygous for the ‘null’ allele (li) have no linamarase activity in this tissue. The isolation of a cDNA clone from linamarase mRNA is described. The cDNA clone is used to further characterise alleles of the Li locus. Northern blot analysis shows that plants homozygous for the ‘null’ allele (li li) produce very reduced levels of mRNA which hybridises to the cDNA. Heterozygous plants (Li li), which have intermediate levels of enzyme activity, produce intermediate levels of mRNA. Southern blot analysis of Hind III digested genomic DNA shows that the white clover genome contains three genes with homology to the linamarase cDNA and that at least two of these genes segregate independently. Analysis of the cosegregation of linamarase activity and the presence of genomic restriction fragments identifies the genomic sequence specifying linamarase structure and indicates either a structural or cis acting control function of the Li locus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00028776
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  • 3
    Electronic Resource
    Electronic Resource
    Nucleotide and derived amino acid sequence of the cyanogenic β-glucosidase (linamarase) from white clover (Trifolium repens L.) (1991)
    Springer
    Plant molecular biology 17 (1991), S. 209-219 
    Details
    ISSN: 1573-5028
    Keywords: β-glucosidase ; cyanogenesis ; linamarase ; Trifolium repens ; white clover ; DNA sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The nucleotide sequence and derived amino acid sequence of two different β-glucosidase cDNA clones were determined. One clone (TRE104) was identified as the cyanogenic β-glucosidase by homology with the N-terminal and internal peptide amino acid sequence of the purified enzyme. The biological function of the other β-glycosidase (TRE361) is not known. Co-segregation of genomic restriction fragments uniquely identified by each cDNA clone shows that these two genes are linked in the white clover genome. Both TRE104 and TRE361 fragments co-segregate with cyanogenic β-glucosidase activity. Extensive homology was found between the white clover β-glucosidase sequences and a group of prokaryote and mammalian β-glycosidases. This group of sequences has no homology with a separate set of β-glucosidase genes isolated from fungi and the thermophilic bacterium Clostridium thermocellum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039495
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  • 4
    Electronic Resource
    Electronic Resource
    A study of dominance at the locus controlling cyanoglucoside production in Trifolium repens L. (1982)
    Springer
    Euphytica 31 (1982), S. 477-483 
    Details
    ISSN: 1573-5060
    Keywords: Trifolium repens ; white clover ; cyanogenesis ; cyanoglucosides ; linamarin ; lotaustralin ; dominance ; gene dosage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The Ac allele for cyanoglucoside production in white clover has been shown to be incompletely dominant and a dosage effect is indicated at this locus. The recessive allele, ac, when homozygous gives rise to absence of cyanoglucosides in the leaf tissue, whereas in heterozygous plants it has been shown to reduce the level of cyanoglucoside, to a level less than half the value found in Ac Ac plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021666
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