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  • Embryonic development  (1)
  • Guinea pig  (1)
  • Transmission electron microscopy  (1)
  • 1
    Electronic Resource
    Electronic Resource
    The presence of transverse and axial tubules in the ventricular myocardium of embryonic and neonatal guinea pigs (1976)
    Springer
    Cell & tissue research 166 (1976), S. 83-90 
    Details
    ISSN: 1432-0878
    Keywords: Embryonic development ; Ventricular myocardium ; Transverse and axial tubules ; Guinea pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Developing transverse (T) tubules are found in embryonic guinea pig ventricular myocardium after approximately eight weeks of gestation. By the time of birth (nine weeks total gestation), longitudinally-oriented axial tubules connected to the T tubules also have formed, and the majority of cells closely resemble those of the adult. The form taken by the developing T and axial tubules suggests that they are generated in a manner similar to that for T tubules in chick and rat skeletal muscle, namely by repeated formation of caveolae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215127
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The preparation of cultured cells of vascular origin for transmission electron microscopy (1984)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 1 (1984), S. 271-277 
    Details
    ISSN: 0741-0581
    Keywords: Vascular cell cultures ; Transmission electron microscopy ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: A method is described for obtaining optimal, reproducible ultrastructure of vascular smooth muscle cells and vascular endothelial cells in culture. Routinely grown cultures are prepared for TEM with a precise regimen of fixation, postfixation, en bloc staining, dehydration, and embedment. The most important aspects of this procedure are the following: (1) fixation with a percentage-gradient series of glutaraldehyde solutions at 37°C, (2) immediate postfixation with osmium tetroxide solution, and (3) block-staining with uranyl acetate solution to eliminate any extraction of constituents during subsequent processing.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060010305
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    Paper (German National Licenses)
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