ISSN:
0935-6304
Keywords:
Thin-layer chromatography, TLC, HPTLC
;
Photometric scanning
;
Radiation transfer equation
;
Multilayer model (10 layers)
;
Remission
;
Transmission
;
Sample concentration
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Quantitative evaluation of TLC by direct photometric scanning is a promising method, in view of the development of cheap microprocessors: soon instruments for digital scanning will be commercialy available. In this paper we describe a multilayer model which seems to be a good approximation for solving the radiation transfer equation in the case of layer models with a gradient in an inward direction. Changes of remitted and transmitted light, in cases where a band lies in different layers, are presented. Examples shown are: the absorbance profile of a chromatographed band in the direction of scanning is approximated to that of an isosceles triangle; the concentration of the band is uniform, e.g. 0.3 A. U.; the concentration of the band is changing from 0 to 2.0 A. U. These results will serve to set up the correct algorithms for digital scanning and simultaneous determination in TLC.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240020806
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