ISSN:
1432-203X
Keywords:
Key words Agrobacterium-mediated transformation
;
B.t. insecticidal protein gene
;
B. campestris ssp.parachinensis
;
Transgenic plants
;
Insect resistance
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract An effective plant regeneration procedure and a gene transfer system via Agrobacterium tumefaciens were developed in Brassica campestris ssp. parachinensis. Hypocotyls from 5-day-old seedlings with 2 days pre-culture were infected with Agrobacterium strain MOG301 harboring a binary vector containing a synthetic Bacillus thuringiensis (B.t.) cry1Ab or cry1Ac gene with full codon-modification. After culture and selection on MS medium supplemented with 4.0 mg/l BAP, 2.0 mg/l NAA, 70 μM AgNO3 and 50 mg/l kanamycin, a number of kanamycin-resistant plantlets were regenerated. PCR and Southern blotting analysis were used to identify and characterize the transgenic plants with the integrated cry1Ab or cry1Ac gene. Western blotting analysis of the transgenic plants confirmed the expression of insecticidal proteins encoded by cry1Ab or cry1Ac. Subsequent bioassay with larvae of the Diamondback moth, Plutella xylostella, demonstrated that the transgenic plants were resistant to feeding damage.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050007
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