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  • Cytochrome b  (1)
  • Transformation of Schizosaccharomyces pombe  (1)
  • 1
    Electronic Resource
    Electronic Resource
    r-DNA plasmid from Schizosaccharomyces pombe: Cloning and use in yeast transformation (1982)
    Springer
    Current genetics 6 (1982), S. 31-38 
    Details
    ISSN: 1432-0983
    Keywords: Ribosomal DNA homology ; Transformation of Schizosaccharomyces pombe ; Restriction mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. an extrachromosomal, extramitochondrial DNA circle of about 3 μm length, occurring in S. pombe, was cloned in pBR322 and a detailed restriction map of the cloned sequence constructed. 2. Hybridization of this DNA with the ribosomal DNA from S. cerevisiae revealed regions of homology which were mapped in the cloned S. pombe DNA. 3. A transformation system was developed using the URA3 gene of S. cerevisiae and the 3 μm molecule. It was shown that the r-DNA part of the hybrid plasmid promotes replication in S. pombe.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00397639
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular cloning of the yeast nuclear genes CBS1 and CBS2 (1986)
    Springer
    Current genetics 11 (1986), S. 47-53 
    Details
    ISSN: 1432-0983
    Keywords: Cytochrome b ; Mitochondria ; Pet genes ; Translation control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The yeast nuclear genes CBS1 and CBS2 are both required for translation of the mitochondrial COB transcripts. Here we report on the identification of two unique chromosomal DNA-sequences of 2 kb and 2.3 kb from yeast wild type gene banks which functionally complement cbs1 and cbs2 mutants, respectively. Disruption of the homologous DNA-fragments by insertion of the URA3 gene generates respiratory deficient cells which fail to complement the original mutants. Cells with these gene disruptions are phenotypically identical to the original cbs1 and cbs2 mutants with respect to cytochrome spectra and mitochondrial translation products. The results exclude the possibility that suppressor genes have been cloned and confirm the conclusion that both genes, CBS1 and CBS2, specifically are involved in translation of mitochondrial COB RNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00389425
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    Paper (German National Licenses)
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