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  • 1
    Electronic Resource
    Electronic Resource
    Transformation of Trichoderma species with dominant selectable markers (1992)
    Springer
    Current genetics 21 (1992), S. 23-26 
    Details
    ISSN: 1432-0983
    Keywords: Trichoderma ; Transformation ; Hygromycin B resistance ; Benomyl resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have developed a transformation system for Trichoderma hamatum and Trichoderma harzianum Rifai, using dominant markers for selection based on the Escherichia coli hygromycin B phosphotransferase gene (hph) and the β-tubulin gene (bml) from Neurospora crassa, respectively. Transformation frequencies and protoplast regeneration were low in both species. All the T. hamatum hygromycin-resistant transformants analysed were mitotically stable, in contrast to those of T. harzianum derived by benomyl resistance, in which only 50% of the transformants analysed were stable. Molecular analysis of transformants showed the integration of the transforming DNA into the genome and indicated that the number and sites of integration varied among the transformants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318649
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  • 2
    Electronic Resource
    Electronic Resource
    Cloning of an Aspergillus niger invertase gene by expression in Trichoderma reesei (1993)
    Springer
    Current genetics 24 (1993), S. 53-59 
    Details
    ISSN: 1432-0983
    Keywords: Aspergillus niger ; Trichoderma reesei ; Invertase ; Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The filamentous fungus Aspergillus niger produces two glycosylated forms of the sucrose-hydrolysing enzyme, invertase. In contrast, some Trichoderma species lack invertase and are unable to utilise sucrose as a sole carbon source. Using an A. niger genomic library constructed in a cosmid vector containing the ura5 gene of Podospora anserina as a selectable marker, and the T. reesei ura5- strain as a sucrose-minus recipient strain, an A. niger invertase gene (suc1) has been cloned by a sib selection procedure. PAGE and enzyme analysis confirmed that transformants had acquired invertase activity. The cloned gene contained DNA sequences which were complementary to the amino-acid sequences of tryptic peptides found in invertase purified from A. niger. The suc1 invertase gene can be used as a dominant selectable marker for the transformation of Trichoderma strains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00324665
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