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  • 78.70.Dm  (2)
  • Transcription  (2)
  • Springer  (4)
  • American Chemical Society
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  • Springer  (4)
  • American Chemical Society
Erscheinungszeitraum
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    The European physical journal 94 (1994), S. 1-2 
    ISSN: 1434-6036
    Schlagwort(e): 79.60.Cn ; 78.70.Dm
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract We report on comparative study of angle-resolved photoelectron spectra of Ni metal and CeGd at theM III andN IV,V soft-x-ray absorption threshold, respectively. OnM III resonance, the valence-band photoemission (PE) intensity of Ni follows a cos2-behavior, while the intensity of the 6-eV satellite is independent of the angle of emission relative to the E-vector of the incoming beam. On the other hand, the 4f Ce PE signal behaves ∝ cos2 onN IV,V resonance. This clearly shows that the resonant enhancement of the 6-eV PE satellite of Ni is mainly caused by an incoherent Auger decay and cannot be described as a resonant PE process.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    ISSN: 1617-4623
    Schlagwort(e): Transcription ; Rap1p ; Gcr1p ; PGK ; Upstream activating sequence
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Analysis of the upstream activation sequence (UAS) of the yeast phosphoglycerate kinase gene (PGK) has demonstrated that a number of sequence elements are involved in its activity and two of these sequences are bound by the multifunctional factors Rap1p and Abftp. In this report we show by in vivo footprinting that the regulatory factor encoded by GCR1 binds to two elements in the 3′ half of the PGK UAS. These elements contain the sequence CTTCC, which was previously suggested to be important for the activity of the PGK UAS and has been shown to be able to bind Gcrlp in vitro. Furthermore, we find that Gcr1p positively influences PGK transcription, although it is not responsible for the carbon source dependent regulation of PGK mRNA synthesis. In order to mediate its transcriptional influence we find that Gcrtp requires the Rap1p binding site, in addition to its own, but not the Abf1p site. As neither a Rapip nor a Gcr1p binding site alone is able to activate transcription, we propose that Gcr1p and Rapip interact in an interdependent fashion to activate PGK transcription.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    ISSN: 1617-4623
    Schlagwort(e): Transcription ; Co-activator ; GAL11 ; RAP1
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Transcription of the yeast phosphoglycerate kinase gene (PGK) is activated by an array of nuclear factors including the multifunctional protein RAP1. We have demonstrated that the transcriptional co-activator GAL11, which was identified as an auxiliary factor to GAL4 and which is believed to interact with the zinc finger of the trans-activator, positively influences the level of PGK transcription on both fermentable and non-fermentable carbon sources. This positive effect is only observed when the RAP1 site in the upstream activation sequence (UAS) is present, implying that GAL11 acts through RAP1. Expression of the RAP1 gene is not reduced in the gal11 background, and in vivo footprinting shows that GAL11 does not influence RAP1 DNA-binding activity. Therefore the effect of GAL11 on PGK transcription must be mediated at the PGK UAS, presumably as part of the activation complex. It has been proposed that RAP1 may act as a facilitator of GCR1 binding at the PGK UAS and therefore it is conceivable that the target for GAL11 may in fact be GCR1. A further implication of this study is that GAL11 can interact with proteins such as RAP1 or GCR1 that are apparently structurally dissimilar from GAL4 and other zinc finger DNA-binding proteins.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    The European physical journal 95 (1994), S. 9-12 
    ISSN: 1434-6036
    Schlagwort(e): 74.70.Vy ; 78.70.Dm ; 79.60.Eq
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract Resonant photoemission studies have been performed at the 2p soft-x-ray absorption thresholds of Fe, Co, and Ni metal. At resonance, the photoelectron intensity exhibits a strong enhancement in the region of the two-hole satellites, which is shown, however, to be mostly due to an incoherent superposition of a photoemission signal with a more intense signal from anL 3 M 4,5 M 4,5 Coster-Kronig decay of the core-ionized state. The resonant enhancement of the valence-band photoemission satellite in Ni metal amounts to a factor of ≃12. In contrast, no photoemission satellites are observed for Fe and Co metal.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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