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  • 1
    Electronic Resource
    Electronic Resource
    Sertoli cells of adult rats “in vitro” (1977)
    Springer
    Cell & tissue research 176 (1977), S. 69-90 
    Details
    ISSN: 1432-0878
    Keywords: Testis ; Sertoli cell ; Cell culture ; Estradiol production
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A cell line obtained from isolated seminiferous tubules of adult rat testis has been studied “in vitro” over a period of 35 days. Light and electron microscopic studies performed from hour 2 to the end of culture have shown the presence of a monomorphic cell population. After 5–6 days of culture the cells formed a monolayer. The cytoplasm of the cells contained numerous lipid bodies and produced numerous projections. The nucleus showed several indentations and one or more nucleoli. From the 9th to the 15th day of culture the cells developed a large amount of endoplasmic reticulum, Golgi apparatus and aggregates of electron dense granules. From the 20th to 40th day the cell cultures progressively degenerated. Immunochemical analysis of the culture medium revealed the presence of estradiol-17β, which reached its maximum production rate from the 8th day to the 18th day of culture. Corresponding to cell involution estradiol concentration underwent a rapid decrease. On the basis of morphological and biochemical data the cells could be considered Sertoli cells. This work was supported by Grants n.∘ 74.00155.04 and n.∘ 75.01224.04 from the Consiglio Nazionale delle Ricerche (C.N.R.), Rome, Italy, and by Istituto di Ricerca F. Angelini, Rome, Italy
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220345
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  • 2
    Electronic Resource
    Electronic Resource
    Immunochemical localization of contractile proteins in mammalian meiotic chromosomes (1980)
    Springer
    Cell & tissue research 213 (1980), S. 159-178 
    Details
    ISSN: 1432-0878
    Keywords: Testis ; Meiotic nuclei ; Myosin ; Actin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Smooth muscle heavy myosin and actin have been detected in mouse and rat meiotic chromosomes, by indirect immunofluorescence performed on testis cryostat sections and isolated germ cells. Both contractile proteins are detectable in the nuclei of meiotic cells during the first prophase. The appearance and disappearance time of myosin and actin, however, is not synchronous. While actin is visible in small spots from resting to late diplotene spermatocytes, myosin appears as filaments in the primary spermatocytes from the zygotene to the early stage of diplotene. The number of myosin filaments in the pachytene spermatocytes corresponds to the number of bivalent chromosomes, whereas actin spots constantly outnumber the pairing chromosomes by two units. These immunochemical observations suggest that the two contractile proteins are associated with the synaptonemal complex (SC). Myosin seems to be associated with the central region of the SC, while actin is present in its basal knob which is in connection with the nuclear membrane. The difference in number between myosin filaments and actin spots appears to be related to the peculiar behaviour of the pairing sex chromosomes. The presence of contractile proteins in the nuclei of primary spermatocytes seems to suggest that they might play a role in the process of pairing of homologous chromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236928
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  • 3
    Electronic Resource
    Electronic Resource
    Morphological, histochemical and biochemical studies on germ cell mitochondria of normal rats (1979)
    Springer
    Cell & tissue research 196 (1979), S. 1-22 
    Details
    ISSN: 1432-0878
    Keywords: Testis ; Germ cells ; Mitochondria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Morphological changes in rat germ cell mitochondria are described. In diplotene and secondary spermatocytes and in the spermatids of the Golgi, cap and acrosomal phases, the mitochondria take on a rounded appearance with the inner space containing the matrix flattened against the outer membrane and the intracristal spaces considerably swollen (“condensed” mitochondria). Functional studies on “condensed” mitochondria isolated from the germ cells of normal rats have been performed. The following parameters have been evaluated: ADP/O ratio, respiratory control ratio (RCR) and ADP affinity. The ADP/O values found in the presence of various substrates are in agreement with the theoretical figures. The RCR is remarkably high. Moreover, the ADP affinity of these mitochondria is very high, as demonstrated by the low values of the “apparent Km”. These biochemical findings, which demonstrate a high oxidative capacity coupled with a marked phosphorylation, suggest that the “condensed” appearance of germ cell mitochondria is the expression of an active functional state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236345
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