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  • 1
    Electronic Resource
    Electronic Resource
    Oviposition-deterring pheromone inRhagoletis cerasi L.: Purification and determination of the chemical constitution (1987)
    Springer
    Cellular and molecular life sciences 43 (1987), S. 157-164 
    Details
    ISSN: 1420-9071
    Keywords: Tephritidae ; Rhagoletis cerasi L ; oviposition-deterring pheromone ; constitution ; purification ; electrophysiology ; contactchemoreceptors ; oviposition behavior ; fast atom bombardment mass spectrometry ; two-dimensional mass spectrometry ; gas chromatography ; mass spectrometry ; 1H-NMR spectroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An oviposition-deterring pheromone (ODP) of the European cherry fruit flyRhagoletis cerasi L. was isolated from faeces using cellulose and several reverse phase TLC and HPLC procedures. The biological activity was evaluated by means of behavior tests and by electrophysiological recordings from tarsal contact chemoreceptors. The compound was structurally characterized as a N[15(β-glucopyranosyl)oxy-8-hydroxypalmitoyl]-taurine by spectroscopic means. The configurations of C-8 and C-15 of the fatty acid constituent remain to be established by synthetic work.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01942834
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  • 2
    Electronic Resource
    Electronic Resource
    Host marking pheromone of Rhagoletis cerasi: field deployment of synthetic pheromone as a novel cherry fruit fly management strategy (1992)
    Springer
    Entomologia experimentalis et applicata 65 (1992), S. 141-147 
    Details
    ISSN: 1570-7458
    Keywords: Rhagoletis cerasi ; Tephritidae ; host marking pheromone ; pest management
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We tested the efficacy of synthetic host marking pheromone (HMP) of the European cherry fruit fly (R. cerasi) as a fruit-infestation-reducing-agent in an experimental cherry orchard. Two different pheromone deployment strategies were compared: covering entire tree canopies with synthetic HMP or treating only one half (top to bottom or lower half) of the tree canopy. Pheromone application caused a tenfold reduction in fruit infestation if the entire tree canopy was covered (0.226 vs 0.021 pupae/fruit in untreated and treated trees, respectively). Results show, nevertheless, that a significant reduction in fruit infestation can be achieved by treating only one half of tree canopies (top to bottom) (0.021 vs 0.048 pupae/fruit when comparing totally vs partially treated trees). We conclude that synthetic cherry fruit fly HMP has potential as a fruit fly management tool, especially in cases where ‘organically’ grown fruit reaches high market values.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221364
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  • 3
    Electronic Resource
    Electronic Resource
    Oviposition deterring pheromone in Rhagoletis cerasi: Behavioral laboratory test to measure pheromone activity (1985)
    Springer
    Entomologia experimentalis et applicata 39 (1985), S. 163-169 
    Details
    ISSN: 1570-7458
    Keywords: Rhagoletis cerasi ; Diptera ; Tephritidae ; oviposition deterring pheromone ; behavior test ; purification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Nach einer einleitenden Übersicht über den heutigen Stand der Erkenntnisse auf dem Gebiet der eiablage-hemmenden Pheromone (ODP) bei Fruchtfliegen und insbesondere der Kirschenfliege wird der verbesserte Verhaltenstest beschrieben, welcher für den halb-quantitativen Nachweis von ODP-Aktivität entwickelt worden ist. Im laufenden interdisziplinären Forschungsprogramm an der Eidg. Forschungsanstalt Wädenswil wird versucht, durch enge Zusammenarbeit zwischen Biologe, Chemiker und Elektrophysiologe das ODP der Kirschenfliege möglichst mit geringem Anteil an Verunreinigung einzusammeln, zu reinigen und einer chemischen Identifikation der ODP Komponenten zugänglich zu machen. Die Möglichkeiten und Grenzen des Verhaltenstests werden diskutiert und die Empfindlichkeit des Verfahrens mittels einer Konzentrationsreihe von Pheromonextrakt aufgezeigt.
    Notes: Abstract Current investigations concerning the identification of the chemical nature of the oviposition deterring pheromone of the European cherry fruit fly, Rhagoletis cerasi L., are conducted in an interdisciplinary research program by combining chemistry with behavioral evaluation. The methods used to collect and evaluate the pheromone by an improved semi-quantitative behavior test are described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00414504
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  • 4
    Electronic Resource
    Electronic Resource
    Oviposition deterring pheromone in Rhagoletis cerasi: Biological activity of a pure single compound verified in semi-field test (1987)
    Springer
    Entomologia experimentalis et applicata 45 (1987), S. 17-22 
    Details
    ISSN: 1570-7458
    Keywords: Rhagoletis cerasi ; Diptera ; Tephritidae ; oviposition deterring pheromone ; behavior ; semi-field bioassay ; electrophysiology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Mit zunehmenden Reinigungsschritten am Rohextrakt des Markierungspheromones (ODP) der Kirschenfliege, Rhagoletis cerasi L., und zunehmenden Reinheitsgrad des aktiven Prinzipes nimmt die Menge an Substanz ab, welche sowohl für die chemische Analyse wie auch die Prüfung der biologischen Aktivität zur Verfügung steht. Beim Vorliegen einer nicht mehr wägbaren Menge eines reinen Stoffes im Sommer 1985, welcher als ein Palmityl-glucopyranosid identifiziert werden konnte (Hurter et al., 1987), musste deshalb auf den üblichen Verhaltenstest im Labor verzichtet und ein neuer Lösungsansatz gefunden werden. Nachdem ein erster elektrophysiologischer Test positive Resultate gezeight hatte, wurde die Fraktion Nr. 2634 in einem grossen Freilandkäfig mit Kirschenpflanzen und natürlichen Früchten in einem Verhaltenstest auf ihre biologische Wirksamkeit geprüft. Die in dieser Arbeit präsentierten Resultate zeigen nicht nur, dass mit dem Palmityl-glucopyranosid eine ähnliche Wirkung erzielt wurde wie mit natürlichen ODP, sondern zum erstenmal konnte demonstriert werden, dass in R. cerasi und möglicherweise in andern Fruchtfliegenarten eine einzelne chemische Substanz eine eiablagehemmende Wirkung ausüben kann.
    Notes: Abstract A pure single compound, a palmityl-glucopyranoside identified as component of the oviposition deterring pheromone (ODP) of the European cherry fruit fly was evaluated with respect to its biological activity by means of a semi-field bioassay in a large field-cage with host plants. The comparison of the observed behaviors of flies exposed to clean cherries, to cherries treated with a standard ODP solution and with the pure compound revealed that palmityl-glucopyranoside elicited the same response as did the crude ODP solution. Irritation indices are described that seem to be of use for the characterization of cherry fruit fly behavior in the presence and absence of ODP. The advantages of the field-cage test over the standard laboratory tests are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00343620
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  • 5
    Electronic Resource
    Electronic Resource
    Host marking pheromone ofRhagoletis cerasi: Foraging behavior in response to synthetic pheromonal isomers (1992)
    Springer
    Journal of chemical ecology 18 (1992), S. 1299-1311 
    Details
    ISSN: 1573-1561
    Keywords: Rhagoletis cerasi ; Diptera ; Tephritidae ; foraging behavior ; host marking pheromone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract We studied the foraging behavior ofRhagoletis cerasi females in trees treated with synthetic cherry fruit fly host marking pheromone (HMP) under seminatural conditions (potted trees enclosed in a screen cage). Results show that synthetic HMP (particularly the 8RS-@#@ 15R isomer configuration (racemic mixture)) was highly effective in eliciting behavioral responses similar to those reported in studies using natural HMP. Flies exposed to synthetic pheromone exhibited short tree residence times (i.e., emigrated faster), increased flight frequency rates (measured as number of alightings per/minute), higher irritation indices while on a tree or a fruit, and oviposited fewer eggs per fruit visit than flies exposed to clean trees and fruit (not treated with synthetic HMP). Furthermore, we provide evidence showing that when flies were continuously exposed to an HMP-saturated environment, they exhibited an increased tendency to lay eggs in marked fruit.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00994357
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