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  • 1
    Electronic Resource
    Electronic Resource
    Chemical shift as a probe of molecular interfaces: NMR studies of DNA binding by the three amino-terminal zinc finger domains from transcription factor IIIA (1998)
    Springer
    Journal of biomolecular NMR 12 (1998), S. 51-71 
    Details
    ISSN: 1573-5001
    Keywords: binding ; chemical shift ; complex ; DNA ; structure ; TFIIIA ; Zinc Finger
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract We report the NMR resonance assignments for a macromolecular protein/DNA complex containing the three amino-terminal zinc fingers (92 amino acid residues) of Xenopus laevis TFIIIA (termed zf1-3) bound to the physiological DNA target (15 base pairs), and for the free DNA. Comparisons are made of the chemical shifts of protein backbone1 HN, 15N,13 Cα and13 Cβ and DNA base and sugar protons of the free and bound species. Chemical shift changes are analyzed in the context of the structures of the zf1-3/DNA complex to assess the utility of chemical shift change as a probe of molecular interfaces. Chemical shift perturbations that occur upon binding in the zf1-3/DNA complex do not correspond directly to the structural interface, but rather arise from a number of direct and indirect structural and dynamic effects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008290631575
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  • 2
    Electronic Resource
    Electronic Resource
    Root:shoot ratios of old and modern, tall and semi-dwarf wheats in a mediterranean environment (1990)
    Springer
    Plant and soil 122 (1990), S. 89-98 
    Details
    ISSN: 1573-5036
    Keywords: harvest index ; old and modern wheats ; root:shoot ratio ; Rht genes ; root dry matter ; root length ; water use efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A field study tested the hypothesis that modern wheat varieties invest a lesser proportion of the total dry matter (root plus shoot) in the root system compared to old varieties. The study was carried out on a duplex soil (sand over clay) at Merredin, Western Australia in a Mediterranean type environment. We also compared the root:shoot dry matter ratios of near-isogenic lines forRht dwarfing genes. Root:shoot ratios decreased with crop growth stage and were closely related to the developmental pattern of a variety. All varieties appeared to accumulate more dry matter into shoots after the terminal spikelet stage. For the modern variety Kulin this occurred as early as 55 days after sowing (DAS), but did not occur until 90 DAS in the old variety Purple Straw. For all varieties, root dry matter reached its maximum at anthesis, while shoot dry matter continued to increase till maturity. At anthesis there were no significant differences in shoot dry matter between varieties, but from Purple Straw to Kulin root dry matter and thus root:shoot ratio decreased. The tall and dwarf isogenic lines had similar developmental and root:shoot dry matter accumulation patterns. At anthesis, the old variety Purple Straw had significantly higher root dry matter and root length density in the top 40-cm of the profile than modern variety Kulin. There were no varietal differences in rooting depth, water extraction or water use. At maturity about 30% of the total dry matter was invested in the roots among wheat varieties. Grain yield, harvest index (HI) and water use efficiency of grain (WUEgr) increased from old to modern varieties. The reduced investment of dry matter in the root system and thus the lower root:shoot ratio from early in the growing season may partly explain the increased HI and WUEgr of modern compared to old varieties.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02444108
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  • 3
    Electronic Resource
    Electronic Resource
    Root:shoot ratios of old and modern, tall and semi-dwarf wheats in a mediterranean environment (1990)
    Springer
    Plant and soil 121 (1990), S. 89-98 
    Details
    ISSN: 1573-5036
    Keywords: harvest index ; old and modern wheats ; root:shoot ratio ; Rht genes ; root dry matter ; root length ; water use efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A field study tested the hypothesis that modern wheat varieties invest a lesser proportion of the total dry matter (root plus shoot) in the root system compared to old varieties. The study was carried out on a duplex soil (sand over clay) at Merredin, Western Australia in a Mediterranean type environment. We also compared the root:shoot dry matter ratios of near-isogenic lines for Rht dwarfing genes. Root:shoot ratios decreased with crop growth stage and were closely related to the developmental pattern of a variety. All varieties appeared to accumulate more dry matter into shoots after the terminal spikelet stage. For the modern variety Kulin this occurred as early as 55 days after sowing (DAS), but did not occur until 90 DAS in the old variety Purple Straw. For all varieties, root dry matter reached its maximum at anthesis, while shoot dry matter continued to increase till maturity. At anthesis there were no significant differences in shoot dry matter between varieties, but from Purple Straw to Kulin root dry matter and thus root:shoot ratio decreased. The tall and dwarf isogenic lines had similar developmental and root:shoot dry matter accumulation patterns. At anthesis, the old variety Purple Straw had significantly higher root dry matter and root length density in the top 40-cm of the profile than modern variety Kulin. There were no varietal differences in rooting depth, water extraction or water use. At maturity about 30% of the total dry matter was invested in the roots among wheat varieties. Grain yield, harvest index (HI) and water use efficiency of grain (WUEgr) increased from old to modern varieties. The reduced investment of dry matter in the root system and thus the lower root:shoot ratio from early in the growing season may partly explain the increased HI and WUEgr of modern compared to old varieties.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00013101
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  • 4
    Electronic Resource
    Electronic Resource
    1H, 15N and 13C resonance assignments for the first three zinc fingers of transcription factor IIIA (1994)
    Springer
    Journal of biomolecular NMR 4 (1994), S. 433-454 
    Details
    ISSN: 1573-5001
    Keywords: TFIIIA ; Zinc finger ; NMR ; Structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The first three zinc fingers (ZF1-3) of transcription factor IIIA (TFIIIA) from Xenopus have been shown to contribute the majority of the binding energy to the intact TFIIIA-DNA interaction [Liao et al. (1992) J. Mol. Biol., 223, 857–871]. We have expressed a 92-amino acid polypeptide containing the three N-terminal zinc fingers of TFIIIA. This three-fingered polypeptide has been isotopically labeled with 15N and 13C in E. coli and purified to homogeneity. Assignment of backbone 1H, 15N, aliphatic 1H and 13C and aromatic 1H and 13C resonances of ΔNZF1-3 has been obtained using a combination of single-, double-and triple-resonance multidimensional NMR experiments. The secondary structures for each finger have been determined from NOE connectivities, 3JNHα values and chemical shifts. The results show that each finger folds into a canonical β-sheet-helix zinc finger structural motif, while the linkers adopt an extended structure. The helix between the two histidine ligands in ZF3 is distorted by zinc coordination, to accommodate the presence of four intervening amino acids instead of three as in ZF1 and ZF2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00179350
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