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Analysis of the expression and function of the σB-dependent general stress regulon of Bacillus subtilis during slow growth (1999)

Schweder, T. ; Kolyschkow, Angela ; Völker, Uwe ; [et al.]

Springer

Archives of microbiology 171 (1999), S. 439-443

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ISSN: 1432-072X

Keywords: Key wordsBacillus subtilis ; sigB ; Chemostat ; Glucose limitation ; Slow growth ; Survival

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Glucose-limited continuous cultures were used to analyze σB activity at decreasing growth rates. Expression of the σB-dependent genes gsiB and ctc started to increase at a growth rate of 0.2 h–1, and both genes were induced approximately fivefold at a growth rate of 0.1 h–1 as compared to expression at the maximal growth rate. However, maximal σB activity was only reached when the growth stopped as a result of the exhaustion of the carbon and energy source glucose. During glucose-limited growth, increased expression of the general stress regulon at growth rates below 0.2 h–1 did not provide wild-type cells with a growth advantage over sigB mutants. Instead, expression of the stress regulon seems to constitute a significant burden during glucose-limited growth, resulting in a selective growth advantage of the sigB mutant as compared to the wild-type at a growth rate of 0.08 h–1.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s002030050731

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First steps from a two-dimensional protein index towards a response-regulation map for Bacillus subtilis (1997)

Antelmann, Haike ; Bernhardt, Jörg ; Schmid, Roland ; [et al.]

Weinheim : Wiley-Blackwell

Electrophoresis 18 (1997), S. 1451-1463

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ISSN: 0173-0835

Keywords: Bacillus subtilis ; Two-dimensional polyacrylamide gel electrophoresis ; Protein index ; Stress proteins ; SigmaB ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Chemistry and Pharmacology

Notes: Data on the identification of proteins of Bacillus subtilis on two-dimensional (2-D) gels as well as their regulation are summarized and the identification of 56 protein spots is included. The pattern of proteins synthesized in Bacillus subtilis during exponential growth, during starvation for glucose or phosphate, or after the imposition of stresses like heat shock, salt- and ethanol stress as well as oxidative stress was analyzed. N-terminal sequencing of protein spots allowed the identification of 93 proteins on 2-D gels, which are required for the synthesis of amino acids and nucleotides, the generation of ATP, for glycolyses, the pentose phosphate cycle, the citric acid cycle as well as for adaptation to a variety of stress conditions. A computer-aided analysis of the 2-D gels was used to monitor the synthesis profile of more than 130 protein spots. Proteins performing housekeeping functions during exponential growth displayed a reduced synthesis rate during stress and starvation, whereas spots induced during stress and starvation were classified as specific stress proteins induced by a single stimulus or a group of related stimuli, or as general stress proteins induced by a variety of entrely different stimuli. The analysis of mutants in global regulators was initiated in order to establish a response regulation map for B. subtilis. These investigations demonstrated that the alternative sigma factor σB is involved in the regulation of almost all of the general stress proteins and that the phoPR two-component system is required for the induction of a large part but not all of the proteins induced by phosphate starvation.

Additional Material: 4 Ill.