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  • 1
    Electronic Resource
    Electronic Resource
    Promotion of sporulation by caffeine pretreatment in Saccharomyces cerevisiae (1976)
    Springer
    Archives of microbiology 108 (1976), S. 149-152 
    Details
    ISSN: 1432-072X
    Keywords: Saccharomyces cerevisiae ; Sporulation ; Ribonuclease ; Caffeine ; Cycloheximide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Changes in RNase activity during sporulation of a homothallic diploid strain of Saccharomyces cerevisiae were measured in caffeine-treated and non-treated cells. 1. In caffeine-treated cells soon after the transfer to the sporulation medium a significant increase in RNase activity was observed; in control cells the rise of RNase activity was less and started after a lag period of 5 h. The final activity of RNase was about twice as high in caffeine-treated cells as in control cells. 2. Increase in RNase activity during sporulation was sensitive to cycloheximide in control cells, but insensitive in caffeine-treated cells. 3. RNases from vegetative cells and from sporulating ones are different in their K m values. Relation of the changes in RNase activity to premeiotic DNA synthesis is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00428944
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  • 2
    Electronic Resource
    Electronic Resource
    Inhibition of sporulation by ethidium bromide and its reversal by fermentable sugars inSaccharomyces cerevisiae (1974)
    Springer
    Archives of microbiology 99 (1974), S. 295-305 
    Details
    ISSN: 1432-072X
    Keywords: Yeast ; Sporulation ; ϱ− Mutation ; Ethidium Bromide ; Sugars ; Acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ethidium bromide completely inhibited the sporulation ofSaccharomyces cerevisiae at a concentration which induces respiration-deficient ϱ− mutation by 100%, when added to the sporulation medium. This inhibition was reversed by addition of glucose, mannose or fructose. Other sugars which are fermented less efficiently by the yeast did not reverse the inhibition of sporulation by ethidium bromide. Inhibition of sporulation by acriflavine was also reversed by glucose. However, the inhibition of sporulation by antimycin A and cycloheximide was not reversed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00696244
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  • 3
    Electronic Resource
    Electronic Resource
    Correlation among turnover of nucleic acids, ribonuclease activity and sporulation ability of Saccharomyces cerevisiae (1976)
    Springer
    Archives of microbiology 111 (1976), S. 13-19 
    Details
    ISSN: 1432-072X
    Keywords: Saccharomyces cerevisiae ; Sporulation ; Ribonuclease ; Turnover of nucleic acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The turnover of nucleic acids and changes in ribonuclease activity during sporulation of Saccharomyces cerevisiae were studied. In the sporulating strains, 37–58% of vegetatively synthesized RNA were degraded during the sporulation process. The degree of degradation of vegetative RNA was proportional to the sporulation ability. In the non-sporulating strains, the degradation of vegetative RNA was less than 28% in the sporulation medium. Accompanied by the degradation of vegetative RNA, a ribonuclease activity increased several times during sporulation. We have found a close relation among the sporulation rate, the degree of the degradation of vegetative RNA and the increase in ribonuclease activity in the sporulation medium, using cells of which sporulation ability was repressed by changing the age or carbon source in various degrees.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00446544
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  • 4
    Electronic Resource
    Electronic Resource
    Promotion of sporulation by caffeine pretreatment in saccharomyces cerevisiae (1975)
    Springer
    Archives of microbiology 106 (1975), S. 159-164 
    Details
    ISSN: 1432-072X
    Keywords: Yeast ; Sporulation ; Turnover of RNA and protein ; Premeiotic DNA synthesis ; Commitment ; Readiness
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cells cultured in the presence of caffeine had high sporulation ability. The sporulation-promotive effect of caffeine was studied, special attention being paid upon changes in nucleic acid metabolism. When transferred to a sporulation medium, the breakdown of RNA, the synthesis of protein, RNA and DNA, commitment to sporulation and the appearance of mature asci took place in caffeine-treated cells significantly earlier than in control cells. Commitment to sporulation occurred before the completion of premeiotic DNA synthesis in both caffeine-treated and control cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00446518
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  • 5
    Electronic Resource
    Electronic Resource
    Comparative studies on sporulation-promotive actions of cyclic AMP, theophylline and caffeine in Saccharomyces cerevisiae (1975)
    Springer
    Archives of microbiology 105 (1975), S. 83-86 
    Details
    ISSN: 1432-072X
    Keywords: Yeast ; Sporulation ; Cyclic AMP ; Theophylline ; Caffeine ; Intracellular Cyclic AMP Level
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cyclic AMP, theophylline and caffeine promoted sporulation when added to a presporulation medium containing glucose. Caffeine promoted sporulation even when added to a presporulation medium containing acetate as the carbon source, but cyclic AMP and theophylline did not. Caffeine did not increase the intracellular cyclic AMP level, while theophylling did significantly when added to a presporulation medium containing glucose Caffeine inhibited the vegetative DNA synthesis with little effect on RNA and protein synthesis, resulting in the increase in cell volume, dry weight, and RNA and protein contents, but cyclic AMP and theophylline did not show such effects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00447118
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