ISSN:
0749-503X
Keywords:
Splicing
;
S. cerevisiae
;
RNA2
;
Life and Medical Sciences
;
Genetics
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
Notes:
The rna2-1 mutant Saccharomyces cerevisiae. has a consitional lethal phenotype, accumulating high molecular weight RNAs of intron-conataining nuclear genes at 36°C. The cloned RNA2 gene suppresses this phenotype and the RNA2 gene product has been implicated in RNA splicing. Rabbit antisera have been raised againts an N-terminal synthetic peptide taken from the RNA2 gene DNA sequence data, and against a β-galactosidase/RNA2 gene fusion protein. Both antisera identify that same 97-105 kd protein from S. cerevisiae cell extracts which is consistent with the predicted size of the RNA2 protein (from the 2800 nucleotide transcript size and DNA sequence data).
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/yea.320020105
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