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  • Flow cytometry  (1)
  • Space Transportation and Safety  (1)
  • 2000-2004  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Methods in cell science 22 (2000), S. 217-223 
    ISSN: 1573-0603
    Keywords: Flow cytometry ; Muscle cell ; Porcine ; Transfection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A detailed methodology is described for fluorescence-activated cell sorting (FACS) of porcine muscle cells that have been transfected to express green fluorescent protein (GFP). Cells are liberated from porcine skeletal muscle and primary cultures are transfected with DNA encoding GFP. Primary cultures are subjected to immunocytochemistry using a primary muscle-specific monoclonal antibody followed by incubation with a phycoerythrin-conjugated second antibody. Transfected myoblasts aresorted from fibroblasts using forward angle light scatter and ninety degree light scatter, phycoerythrin fluorescence, and GFP fluorescence. These procedures allow for isolation of genetically-engineered porcine muscle cells more rapidly than traditional clonal selection procedures. Consequently, FACS provides porcine myoblast populations that retain the majority of their replicative capacity and are not contaminated with non-myogenic cells.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2019-07-13
    Description: In response to a NASA request, the National Space Propulsion Synergy Team (SPST) team agreed to provide technical and programmatic support to NASA in formulating a Spaceliner 100 Technology Program. The SPST offered a broad cross-section of expertise and experience. Its membership consists of senior level, volunteer representatives from across government, industry, and academia. The purpose of this paper is to provide a summary of the SPST support of SL100, which culminated in a propulsion technologies assessment and prioritization workshop conducted at MSFC. The results of this workshop and the follow-up analysis are part of this report. Also included, is a review of some "lessons learned" that were solicited from the workshop participants.
    Keywords: Space Transportation and Safety
    Type: AIAA Paper 2000-3603 , Joint Propulsion; Jul 17, 2000 - Jul 19, 2000; Huntsville, AL; United States
    Format: application/pdf
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