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  • Cell & Developmental Biology  (15)
  • MATERIALS, METALLIC  (6)
  • Soil strength  (6)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 95 (1986), S. 123-133 
    ISSN: 1573-5036
    Keywords: Aggregate ; Compaction pan ; Penetrometer ; Root penetration ; Seed-bed ; Soil strength ; Triticum aestivum ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary When elongating seminal roots of developing plants reach the base of a tilled seedbed, they often encounter a layer of dense, strong untilled soil. At this interface, they may be deflected horizontally and instead of penetrating the sub-soil, they may form a horizontal mat of roots at the base of the seed-bed. If this occurs, the plants are unable to absorb the reserves of water in the sub-soil, and are very sensitive to short periods of drought. Model experiments were done with artificial sub-soil layers having a range of strengths and with artificial seed-beds having sieved soil aggregates in the 4–7.7, 2–4 and 1–2 mm size ranges. Roots of wheat were grown through the aggregate beds and the proportions of roots which penetrated into the sub-soil were investigated as functions of sub-soil strength and diameter of the aggregates in the seed-bed. The proportion penetrating was found to decrease exponentially with sub-soil strength. The rate of decrease was similar for the 2–4 and 1–2 mm aggregates but was greater for the 4–6.7 mm aggregates. It is concluded that, provided that the roots of different plant species behave similarly, the base of the seed-bed should be composed of fine aggregates and that the penetrometer strength of the underlying untilled sub-soil should not exceed 0.4 MPa for plants with a single seminal axis or 3 MPa for plants such as wheat with 4 seminal axes.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 79 (1984), S. 11-28 
    ISSN: 1573-5036
    Keywords: Beta distribution ; Buckling ; Cracks ; Crack width ; Lateral roots ; Pea ; Penetration ; Penetrometer ; Rape ; Remoulded soil ; Roots ; Safflower ; Seminal axes ; Soil strength ; Undisturbed soil ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary It is shown that probabilities of root penetration across cracks in soil can be calculated effectively using a mathematical model involving root stress and soil distributions and penetrometer/root stress ratios. Penetration criteria are developed, and it is found that the effective penetrometer/root stress ratios take values of about 4 for crack widths smaller than about 2 mm and about 8 for wider cracks. Root swelling does not appear to contribute significantly to the probability of root penetration through any effect on root buckling stress. Suggestions are made for further work on the effects of soil structure and strength on root behaviour.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 77 (1984), S. 141-149 
    ISSN: 1573-5036
    Keywords: Cracks ; Crack width ; Lateral roots ; Pea ; Penetration ; Penetrometer ; Rape ; Remoulded soil ; Roots ; Safflower ; Seminal axes ; Soil strength ; Undisturbed soil ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Experimental methods are described for observing the behaviour of roots encountering cracks in soil. The proportions of roots which enter a second soil block after crossing a crack of known width were measured. Soil strength was measured with a penetrometer. Results are presented for the proportions of seminal roots of wheat and primary lateral roots of pea which enter moulded soil of various strengths after crossing cracks. Results are also presented for the proportions of seminal roots of pea, rape and safflower which enter undisturbed soil after crossing cracks. It was found that, in all cases, the proportion of roots penetrating the second soil block decreased with increasing crack width and increasing soil strength. Also, a smaller proportion of thinner roots penetrated the second soil block than thicker roots under similar conditions. Root diameter in the cracks was influenced by both crack width and soil strength, and an empirical equation is presented to describe this effect.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 52 (1979), S. 325-343 
    ISSN: 1573-5036
    Keywords: Aggregate size ; Maize ; Nutrient uptake ; Root growth ; Soil strength ; Soil structrure ; Sorghum ; Soybean ; Stochastic model
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The growth of roots of maize, sorghum and soybean is modelled through beds of spherical aggregates. Effects of aggregate size and strength, and effects of the spread or distribution of aggregate strengths are investigated. This is achieved by a combination of a statistical model for soil structure with a statistical model for the penetration behaviour of a root at a void/aggregate interface. It is shown that the behaviour of a root at such an interfac is dependent on the previous history of the root in its passage through the soil. It is concluded that the smaller the aggregate size, the greater is the nutrient availability per unit length of root. The influence of aggregate size decreases with increasing soil strength. An increase in aggregate strength reduces the availability of nutrients per unit length of root. However, the rate of nutrient uptake per root axis goes through a minimum at a strength (for maize) of around 80 per cent of the maximum limiting aggregate strength for root penetration. An increase in the spread of aggregate strengths usually results in a proportional increase in nutrient availability. This effect is more pronounced with smaller aggregate sizes.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 98 (1987), S. 303-312 
    ISSN: 1573-5036
    Keywords: Elongation ; Extensibility ; Mechanical stress ; Osmoregulation ; Soil strength ; Water stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A model is developed for the rate of elongation of a root tip in terms of the balance of pressures acting on the root. Differentials of this equation give expressions for the changes in root elongation rate with respect to soil water potential and soil mechanical resistance. The model predicts that root cells osmoregulate against both water stress and soil mechanical resistance with predicts that root cells osmoregulate against both water stress and soil mechanical resistance with similar efficiencies which are less than 100%. Analysis of published data leads to the conclusion that root tips of pea osmoregulate with 70% efficiency. A working equation is developed for the elongation rate of roots in conditions of combined water stress and mechanical resistance.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 74 (1983), S. 153-162 
    ISSN: 1573-5036
    Keywords: Cracks ; Crack orientation ; Elongation rate ; Pea ; Penetration ; Penetrometer ; Rape ; Roots ; Safflower ; Soil strength ; Soil structure ; Undisturbed soil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Experimental methods are described for observing the behaviour of roots growing over the surfaces of undisturbed soil clods and for roots growing along narrow cracks between two clods. Seminal roots of pea, rape and safflower were compared for a range of soil strengths and angles of inclination of the clod surfaces. For all three plant species, the ability of the roots to penetrate ped surfaces decreased with increasing soil strength and increasing angle of the surface relative to the horizontal. However, there was considerable variability of behaviour between roots. Roots were able to elongate more rapidly in cracks narrower than the root diameter than through undisturbed clods without cracks, provided that the crack was not orientated at an oblique angle to the preferred geotropic growth direction.
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 91 (1977), S. 335-344 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A liquid culture system is described whereby proliferation of haemopoietic stem cells (CFU-S), production of granulocyte precursor cells (CFU-C), and extensive granulopoiesis can be maintained in vitro for several months. Such cultures consist of adherent and non-adherent populations of cells. The adherent population contains phagocytic mononuclear cells, “epithelial” cells, and “giant fat” cells. The latter appear to be particularly important for stem cell maintenance and furthermore there is a strong tendency for maturing granulocytes to selectively cluster in and around areas of “giant fat” cell aggregations. By “feeding” the cultures at weekly intervals, between 10 to 15 “population doublings” of functionally normal CFU-S regularly occurs. Increased “population doublings” may be obtained by feeding twice weekly. The cultures show initially extensive granulopoiesis followed, in a majority of cases, by an accumulation of blast cells. Eventually both blast cells and granulocytes decline and the cultures contain predominantly phagocytic mononuclear cells.Culturing at 33°C leads to the development of a more profuse growth of adherent cells and these cultures show better maintenance of stem cells and increased cell density.When tested for colony stimulating activity (CSA) the cultures were uniformly negative. Addition of exogenous CSA caused a rapid decline in stem cells, reduced granulopoiesis and an accumulation of phagocytic mononuclear cells.
    Additional Material: 4 Ill.
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 102 (1980), S. 279-286 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The effects of L-cell conditioned medium which contains granulocyte/macrophage colony stimulating factor (CSF); of highly purified L-cell CSF; and the antiserum directed against L-cell CSF, have been investigated in long-term murine bone marrow cultures. Treatment of cultures with CSF containing conditioned medium led to a rapid decline in haemopoiesis. However, this inhibition of in vitro haemopoiesis is probably caused by materials other than CSF, since the addition of highly purified L-cell CSF had no appreciable effect upon long-term haemopoietic cell proliferation or differentiation. Furthermore, the inhibitory activity of L-cell conditioned medium was not abrogated following neutralization of the CSF activity by CSF antiserum. The direct addition of CSF antiserum did not inhibit granulocyte or macrophage formation. These results suggest that long-term cultures of murine marrow cells may show extensive interactions with stromal cells which are not influenced by exogenous stimulatory or inhibitory factors.
    Additional Material: 4 Tab.
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 112 (1982), S. 123-127 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Three preadipocyte cell lines that have been independently derived from bone marrow stroma (Lanotte et al, 1982) have been tested for their capacity to produce granulocyte, macrophage, and erythroid colony-stimulating factors (CSFs). All elaborated colony-stimulating material that was active upon adult mouse marrow granulocyte/macrophage colony-forming cells (M-CFC) but not foetal liver GM-CFC. The major activity was characterised as a monocyte-macrophage colony-stimulating factor (M-CSF), and the pattern of colony stimulation was similar to that seen after addition of highly purified L-cell CSF. Furthermore, the stimulating activity was specifically neutralised by rabbit anti-L cell CSF antibodies. No evidence was found for stimulation of multipotential or erythroid colony-forming cells, only few granulocytic colonies were detected, and the stimulating activity had no mouse strain restriction. All cell lines produced large quantities of M-CSF; however, the production was found to be modulated during the adipogenesis process. A peak in M-CSF production corresponded to the period of growth arrest after confluence of the stromal cells was reached and when adipocyte maturation was at an early stage. A marked depression in M-CSF secretion was associated with the final steps of adipocyte maturation.
    Additional Material: 1 Ill.
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  • 10
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 123 (1985), S. 73-78 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: WEHI-3B myelomonocytic leukaemia cells secrete a haemopoietic cell growth factor (HCGF) which facilitates the proliferation and development of multipotential stem cells and committed progenitor cells. Several cloned, nonleukaemic cell lines (FDC-P cells) are absolutely dependent on HCGF and die in the absence of it. In these cell lines, factor dependence is associated with the ability of HCGF to increase glucose uptake, thereby controlling glycolytic flux and intracellular ATP levels. We have now investigated the effects of HCGF on glucose uptake in WEHI-3B cells. At 20°C 2-deoxyglucose uptake could be stimulated by the addition of HCGF to the extracellular medium. L-glucose uptake was markedly lower than 2-deoxyglucose uptake and did not respond to the addition of HCGF. At 37°C no HCGF stimulation of 2-deoxyglucose uptake was found. However, at this temperature HCGF release from WEHI-3B cells was markedly higher than at 20°C. Our experiments indicate that HCGF stimulates the glucose transport system in both WEHI-3 cells and FDC-P cells. The similarities between the WEHI-3B cell and FDC-P2 cell polypeptide phenotype were investigated using two-dimensional isoelectric focussing/poly-acrylamide gel electrophoresis. This revealed a high degree of correlation between the two cell types in their protein constituents, indicating a close relationship between the normal and leukaemic cells. These similarities between WEHI-3B cells and FDC-P2 cells are considered and their relevance to haemopoiesis and leukaemogenesis is discussed.
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