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  • Secretion  (1)
  • Silk  (1)
  • critical electrolyte concentration  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Apoptosis: identification by a critical electrolyte concentration method (1996)
    Springer
    Apoptosis 1 (1996), S. 218-221 
    Details
    ISSN: 1573-675X
    Keywords: Apoptosis ; critical electrolyte concentration ; cytochemistry ; nucleic acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A critical electrolyte concentration method previously proposed to distinguish RNA from DNA was considered for a rapid screening of apoptosis at the cellular level. For the monitoring of the apoptotic phenomenon, preparations subjected to anin situ 3′ end DNA labelling assay were also analyzed. The results indicated that the critical electrolyte concentration assay as used here is a simple and useful tool for the rapid identification of apoptotic cells and could contribute to the determination of their frequency, localization and other characteristics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01321106
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  • 2
    Electronic Resource
    Electronic Resource
    Histochemical and histophysical aspects of silk secretion in Melipona quadrifasciata (Hym., Apoidea) (1971)
    Springer
    Cell & tissue research 118 (1971), S. 555-569 
    Details
    ISSN: 1432-0878
    Keywords: Silk glands ; Melipona quadrifasciata ; Secretion ; Polysaccharides ; Structural protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Saure und neutrale Polysaccharide sind im Seidendrüsen-Sekret von Melipona quadrifasciata während aller Funktionsperioden vorhanden, außer in der späten 5. Periode. Jedoch erst von der 3. Stufe an wird ein molekular-orientiertes Strukturprotein gefunden. Dieses Protein ist nicht Kollagen: 1. weil die optischen Anzeichen seiner Doppelbrechung nicht nach Durchführung der von Ebner-Reaktion umgekehrt werden; 2. weil es sichtbar erst dann sezerniert wird, wenn die Zelle eine für diesen Funktionsablauf optimale Ploidiestufe erreicht hat. Dies wird zuerst im distalen Teil der Drüsen beobachtet und breitet sich dann über das ganze Organ aus.
    Notes: Summary Acid and neutral polysaccharides exist in the secretion of the silk glands of Melipona quadrifasciata during the entire functional stage, except in the late 5th instar. However, only from the 3rd instar on is a molecularly oriented structural protein present. The following evidence indicates that this protein is not collagen: (1) the optical sign of its birefringence does not become reversed after von Ebner's reaction, and (2) its secretion can be detected when the cell nucleus attains a ploidy degree optimal for secretory activity. This is at first observed in the distal part of the glands and then throughout the organ.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00324620
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  • 3
    Electronic Resource
    Electronic Resource
    The salivary gland secretions of a neotropical bumblebee (1979)
    Springer
    Protoplasma 100 (1979), S. 251-265 
    Details
    ISSN: 1615-6102
    Keywords: Bombus atratus ; Salivary gland secretion cytochemistry ; Salivary gland ; Silk
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The larval salivary gland secretions ofBombus atratus were studied with cytochemical and cytophysical methods. In the young feeding larvae a proteic filamentous secretion depicting striations perpendicular to the long axis of its fibrillary threads and exhibiting a wellordered macromolecular array was found. It appears not to differ from the silk secretion described for the fully grown larvae of an europeanBombus species. However, in the fully grown larvae ofB. atratus changes which have not yet been reported for other bees occur involving the salivary gland secretion. Two secretion types are then distinguishable. One is composed of carboxylated and sulfated acid glycosaminoglycans and glycoprotein(s) (mucous secretion), and the other has the same composition as that of the filamentous secretion of the young larvae, although differing morphologically and in terms of macromolecular alignment (flocculent secretion). The filamentous secretion is assumed to be involved inB. atratus with the spinning of the “silken partitions” which at a relatively early stage separate the larvae reared within a common cell from one another. The mucous and flocculent secretions will participate in the cocoons which will cover the pupating larvae. The filamentous and flocculent secretions appear to contain an α-helical fibroin, glycoprotein(s) and lipoprotein(s), but not collagen-type proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279315
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