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  • 1
    Electronic Resource
    Electronic Resource
    Visualization of a self-incompatibility gene in Brassica campestris L. by multicolor FISH (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 751-757 
    Details
    ISSN: 1432-2242
    Keywords: Key words Brassica campestris ; Multicolor ; FISH ; Self-incompatibility ; S-glycoprotein (SLG) gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The physical localization of the S-glycoprotein (SLG) locus in the chromosome of Brassica campestris L. ‘pekinensis’ cv ‘Kukai’ was visualized by multi-color fluorescent in situ hybridization (McFISH). ‘Kukai’, which is an F1 hybrid between two parental lines, T-17 and T-18, has two SLG genes from both T-17 and T-18. In this study, a 1.3-kb DNA fragment was amplified from the genomic DNA of T-17 by PCR using a set of primers specific to the class-I SLG. From the genomic DNA of T-18, no DNA fragment was amplified using these primers. In the genomic Southern hybridization, a cloned PCR product hybridized with the genomic DNA of T-17 or F1 but not with that of T-18. The PCR product had a sequence homology of approximately, 85% to another class-I SLG gene, SLG-9. Therefore, the PCR product from T-17 was named SLG-17, as it is thought to be a member of the class-I SLG. Using SLG-17 as the probe, FISH was carried out to visualize the position of the SLG locus. McFISH was also carried out simultaneously using the SLG-17 and SLG-9 genes as probes. The SLG-17 gene was detected as a doublet signal at the interstitial region close to the end of a small chromosome, with the signal site being identical to that of SLG-9. Therefore, it is concluded that the SLG-17 gene is localized at the interstitial region close to the end of the chromosome derived from T-17 in Brassica campestris L. ‘pekinensis’ cv ‘Kukai’.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050798
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  • 2
    Electronic Resource
    Electronic Resource
    Physical distances between S-locus genes in various S haplotypes of Brassica rapa and B. oleracea (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 80-85 
    Details
    ISSN: 1432-2242
    Keywords: Key words Brassica rapa (syn. campestris) ; B. oleracea ; PFGE ; S-locus ; Self-incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  In Brassica, self-incompatibility genes SLG (for S-locus glycoprotein) and SRK (for S-receptor kinase) are located in the S-locus complex region with several other S-linked genes. The S locus is a highly polymorphic region: polymorphism has been observed not only in sequences of SLG and SRK but also in the location of the S-locus genes. In order to compare the physical location of the S-locus genes in various S haplotypes, we used six class-I S haplotypes of B. rapa and seven class-I S haplotypes of B. oleracea in this study. DNA gel blot analysis using pulsed-field gel electrophoresis (PFGE) showed that the physical distances between SLG and SRK in B. rapa are significantly shorter than those in B. oleracea and that the sizes of MluI and BssHII fragments harboring SLG and SRK are less variable within B. rapa than within B. oleracea. We concluded that several large genomic fragments might have been inserted into the S-locus region of B. oleracea after allelic differentiation of S-locus genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051452
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  • 3
    Electronic Resource
    Electronic Resource
    Localization of an SLG protein expressed under the regulation of a tapetum-specific promoter in anthers of transgenic Brassica napus (1998)
    Springer
    Sexual plant reproduction 11 (1998), S. 245-250 
    Details
    ISSN: 1432-2145
    Keywords: Key words Anther ; Self-incompatibility ; S-locus glycoprotein ; Tapetum-specific promoter ; Transgenic Brassica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  S-locus glycoprotein (SLG) is known to be one of the proteins related to self-incompatibility in Brassica, and its transcripts are detected in anthers as well as stigmas. However, an SLG protein has not been detected in anthers so far. Because of sporophytic control of the self-incompatibility (SI) phenotype of pollen, an SLG gene is expected to be expressed in the sporophytic tissue of anthers, i.e., the tapetum. Overexpression of an SLG gene in the tapetum would enable us to predict the localization and function of an SLG protein in anthers. In this study, an SLG gene of self-incompatible B. campestris under the control of a tapetum-specific promoter was introduced into self-compatible B. napus. Immunoblot analysis using anti-SLG antiserum detected the exogenous SLG protein in the immature anthers, but not in the mature anthers. Immunoelectron microscopy showed the SLG protein to be localized in the tapetum and in the exine cell wall layer at the stage when the tapetum was degenerating. This result indicates the possible movement of the SLG protein from the tapetum to the pollen surface. A pollination test indicated that the pollen of the transgenic B. napus did not gain the SI phenotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050149
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  • 4
    Electronic Resource
    Electronic Resource
    High sequence similarity between SLG and the receptor domain of SRK is not necessarily involved in higher dominance relationships in stigma in self-incompatible Brassica rapa L. (1998)
    Springer
    Sexual plant reproduction 11 (1998), S. 292-294 
    Details
    ISSN: 1432-2145
    Keywords: Key words Brassica rapa ; Dominance relationships ; Self-incompatibility ; SLG ; SRK
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050156
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  • 5
    Electronic Resource
    Electronic Resource
    Molecular characterization of SLG and S-related genes in a self-compatible Brassica campestris L. var. yellow sarson (1997)
    Springer
    Sexual plant reproduction 10 (1997), S. 332-340 
    Details
    ISSN: 1432-2145
    Keywords: Key words Brassica campestris L. var. yellow sarson ; Self-compatibility ; Self-incompatibility ; S-locus glycoprotein (SLG) ; S-locus related genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  From a stigma cDNA library of a self-compatible strain, designated C636, of Brassica campestris var. yellow sarson, we isolated three cDNA clones that showed a high degree of sequence similarity with cDNAs encoding either SLG or SLR’s. These three cDNA clones were designated SLG (C636), SLR1 (C636), and SLR2 (C636), respectively. Restriction fragment length polymorphism (RFLP) linkage analyses of a segregating F2 progeny of a hybrid between C636 and the self-incompatible S 8 -homozygote revealed that SLG (C636) was linked to the S locus, whereas SLR1 (C636) and SLR2 (C636) were not. The latter two genes were not linked to each other. The transcripts of SLG (C636), SLR1 (C636), and SLR2 (C636) were detected in stigmas, but not in anthers, of C636. However, the steady-state level of the SLG (C636) transcript was significantly lower than that of the SLG transcript in the self-incompatible S 9 -homozygote. No SRK transcripts were detected in the stigma tissue of C636, whereas an RNA band of the expected size of the SRK transcript was detected in the self-incompatible S 9 -homozygote. The SLG protein was detected in C636 and in three other self-compatible yellow sarson strains by immunoblot analysis; however, the amounts were lower than those of SLGs in self-incompatible strains. We conclude that one reason for the breakdown of self-compatibility in C636 yellow sarson is the down-regulation of the SLG gene and/or failure of the expression of the SRK gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050107
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  • 6
    Electronic Resource
    Electronic Resource
    Two anther-expressed genes downstream of SLG 9 : identification of a novel S-linked gene specifically expressed in anthers at the uninucleate stage of Brassica campestris (syn. rapa) L. (1999)
    Springer
    Sexual plant reproduction 12 (1999), S. 127-134 
    Details
    ISSN: 1432-2145
    Keywords: Key words Anther-expressed gene ; Brassica campestris (syn. rapa) L. ; Self-incompatibility ; SLG ; S locus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The sporophytic self-incompatibility (SI) system in Brassica campestris (syn. rapa) is controlled by multiple alleles of a single locus, S, in which SLG and SRK are located. SLG and/or SRK appear to comprise the female component of the recognition reaction of SI. The gene encoding the male component must also be located at the S locus but has not been identified to date. RNA gel blot analysis with regions flanking SLG 9 and SRK 9 revealed two anther-expressed genes 3’ of the SLG 9 gene. Two cDNA clones were isolated using the 3’-flanking region of SLG 9 as probe. Because of sequence similarity with the Brassica SLL2 gene, one cDNA clone was designated SLL2-S 9 (for S Locus-Linked 2-S 9 gene). The 5’-region of SLL2-S 9 was not present in other SLL2 genes examined. The second gene was novel and was designated SAE1-S 9 (for S locus Anther Expressed 1-S 9 gene). SAE1-S 9 and SLL2-S 9 were located approximately 2.5 kb and 5.5 kb, respectively, downstream of SLG 9 . A 1.4-kb transcript for SLL2-S 9 was detected in both vegetative and reproductive tissues, whereas 1.9-kb and 2.4-kb transcripts were specifically detected in the anther of S 9 haplotype. SAE1-S 9 was specifically expressed in the anther at the uninucleate stage. This gene encoded a novel polypeptide of 257 amino acids. Database analysis revealed a homologous sequence in the upstream region of SRK 3 of B. oleracea. We discuss possible relationships between SLL2-S 9 and SAE1-S 9 and SI.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050183
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