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  • 1
    Publication Date: 2021-05-19
    Description: في هذه الدراسة تمت متابعة الطحلب الأحمر Gelidium spinosum على سواحل المنستير منذ سبتمبر 2000 إلى أوت 2001 و أبرزت النتائج المتحّصل عليها أن الوزن الأقصى تم تسجيله في ماي وهو حوالي 37غ أما الطول الأقصى (11 صم) فقد تم الحصول عليه في شهر جوان . و أما الكتلة الحيوية الأعلى فقد تم التحصل عليها في شهر ماي (حوالي 7 كغ/م2) . أما نسبة الخصوبة فتراوحت بين 0 و 100% . كل هذه النتائج بينت لنا أن الطحلب Gelidium spinosum هو نبتة معمرة لكن حضورها متميز أكثر في الربيع والصيف .
    Description: La rhodophycée Gelidium spinosum (S.G. Gmelin) P.C. Silva (Rhodophycées (Gelidiales .Rhodophyta ) a été étudiée pendant une année, depuis septembre 2000 jusqu’à août 2001 , sur la côte de Monastir (Tunisie) . Les résultats globaux obtenus montrent que le poids humide moyen maximum (environ 37 g /individu) a été atteint en mai. La longueur maximale (environ 11 cm) a été enregistrée en juin. La biomasse maximale (environ 7000g poids humide/ m2) a été obtenue en mai. Quant à la fertilité, elle a varié entre 0%(Mai) et 100% (Septembre, Juin, Juillet et Août) .
    Description: The red algae Gelidium spinosum (Gelidiales .Rhodophyta ) was studied throughout a year, since September 2000 to August 2001,along Monastir coasts (East of Tunisia).Main results showed that the average of wet weight was obtained in May (37 g/ individual).In June, we have recorded the maximum of length (about 11 cm).Wet biomass has reached its maximum in May (7000g wet weight/ m-2 ).The fertility varied between 0 and 100%(September, June, July and August).
    Description: Published
    Keywords: Gelidiales Gelidium spinosum Rhodophyta ; Biomass ; Length ; Fertility ; Seaweeds ; Weight ; Biomass ; Fertility ; Length ; Seaweeds ; Weight
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed , Article
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  • 2
    Publication Date: 2021-05-19
    Description: L'etude d'une population naturelle de l'algue rouge Cystoclonium purpureum a montre que les thalles debutent leur croissonce au printemps pour atteindre leur developpement maximum ent ete. En automne, les algues commencent a disparaitre progressivement. En effet, le poids maximum des thalles est atteint en Juillet (No. 40g). Pendant la meme periode, la longueur des algues atteint son maximum (25,3 cm). Quand au diametre de la fronde, son evolution au cours du temps n'est pas claire. La biomass de Cystoclonium atteint egalement son apogee en Juillet (environ 35 g de MS/m2)
    Description: Study of Cystoclonium purpureum showed that thalli begin to grow in spring and reach highest developement in summer. In autumn, these algae disappear gradually. In fact, the maximum weight is obtained in July (No.40g). In the same period, algae length reach its maximum value, too (25,3cm). While frond diameter evolution isn't clear. Biomass of Cystoclonium reach its highest value in July, too (about 35g DM/m super(2))
    Description: Published
    Keywords: Biomasse ; Rhodophycée ; Growth ; Weight ; Length ; Population dynamics ; Seaweeds ; Biomass ; Seasonal variations ; Cystoclonium purpureum ; Marine
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp. 20-36
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  • 3
    Publication Date: 2021-05-19
    Description: L'etude de l'influence de la temperature et de la lumiere sur la croissance des tetraspores Gracilaria verrucosa a montre que l'intensite lumineuse optimale est comprise entre 85 et 110 Kappa E/m super(2)/s a 20 degree C et entre 80 et 100 Kappa E/ m super(2)/s a 25 degree C
    Description: Study of temperature and light influence on Gacilaria verrucosa tetraspores growth showed that optimal light intensity is situated between 85 and 110 Kappa E/m super(2)/s at 2 degree C and between 80 and 100 Kappa E/m super(2)/s at 25 degree C.
    Description: Published
    Keywords: Croissance ; Tétraspores ; Rhodophycée ; Temperature effects ; Growth ; Light intensity ; Seaweeds ; Gracilaria verrucosa ; Marine
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp. 62-79
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  • 4
    Publication Date: 2021-05-19
    Description: L’estimation de la biomasse de la macroalgue Gracilaria dans le lac nord de Tunis a été réalisée au cours de Juin-Juillet 1996 à partir de 38 stations d’échantillonnage couvrant tous les types de recouvrement de l’espèce. Il en ressort que la biomasse en poids humide est de 191 g/m2 (28 g/m2 en poids sec) et engendre un potentiel total estimé à 508 tonnes, soit 74 tonnes en poids sec (la matière sèche représentant en moyenne 14,5 % de la matière humide). Eu égard à la quantité de Gracilaria disponible à l’état naturel, la valorisation de cette espèce au niveau de ce site pourrait demander le recours à la phycoculture.
    Description: The red seaweed Gracilaria biomass in the Tunis north lake (Tunisia) was estimated between June and July 1996 with reference to different stations. This biomass represent 191 g/m super(2) (wet weight) and 28 g/m super(2) (dry weight). so the total quantity is estimated to 508 t in wet and 74 in dry (the average of dry material represent in general 14,5 % of the humid materiel). Because of its low natural total production, the development of Gracilaria seaweed in this site need culture processing.
    Description: Published
    Keywords: Macroalgues ; Phycoculture ; Potential resources ; Aquaculture development ; Marine aquaculture ; Seaweed culture ; Coastal lagoons ; Biomass ; Seaweeds ; Gracilaria ; Brackish
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: 15-27
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  • 5
    Publication Date: 2021-05-19
    Description: Ce travail concerne la repatition spatiale de la macroalgue Gracilaria dans le lac nord de Tunis et la determination des surfaces d'extension correspondant aux differents types de recouvrement de cette espece, durant les moins de Juin-Juillet 1996. Les peuplements de Gracilaria couvrent 265 ha soit 10% seulement de la surface totale du lac et se presentent sous forme de ceintures dans les profondeurs limitrophes (59,5%) ainsi qu'a l'interieur du lac (24%) et au voisinage de la digue centrale (17%).
    Description: This work concerns the red macro seaweed Gracilaria distribution in the Tunis north lake and the surface corresponding to each type recovering during June and July 1996. The Gracilaria covers 265 ha of the lake which correspond to 10% of it's total surface. 59,5% is surrounding the water plan in shallow water, 24% inside and 17% near the central dyke
    Description: Published
    Keywords: Macroalgues ; Cartographie ; Geographical distribution ; Recovery ; Coastal lagoons ; Seaweeds ; Gracilaria ; Marine
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp 55-72
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  • 6
    Publication Date: 2011-08-24
    Description: A mesophilic toluene-degrading consortium (TDC) and an ethylbenzene-degrading consortium (EDC) were established under sulfate-reducing conditions. These consortia were first characterized by denaturing gradient gel electrophoresis (DGGE) fingerprinting of PCR-amplified 16S rRNA gene fragments, followed by sequencing. The sequences of the major bands (T-1 and E-2) belonging to TDC and EDC, respectively, were affiliated with the family Desulfobacteriaceae. Another major band from EDC (E-1) was related to an uncultured non-sulfate-reducing soil bacterium. Oligonucleotide probes specific for the 16S rRNAs of target organisms corresponding to T-1, E-1, and E-2 were designed, and hybridization conditions were optimized for two analytical formats, membrane and DNA microarray hybridization. Both formats were used to characterize the TDC and EDC, and the results of both were consistent with DGGE analysis. In order to assess the utility of the microarray format for analysis of environmental samples, oil-contaminated sediments from the coast of Kuwait were analyzed. The DNA microarray successfully detected bacterial nucleic acids from these samples, but probes targeting specific groups of sulfate-reducing bacteria did not give positive signals. The results of this study demonstrate the limitations and the potential utility of DNA microarrays for microbial community analysis.
    Keywords: Life Sciences (General)
    Type: Applied and environmental microbiology (ISSN 0099-2240); Volume 68; 7; 3215-25
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  • 7
    Publication Date: 2011-08-24
    Description: Oligonucleotide microarrays were used to profile directly extracted rRNA from environmental microbial populations without PCR amplification. In our initial inspection of two distinct estuarine study sites, the hybridization patterns were reproducible and varied between estuarine sediments of differing salinities. The determination of a thermal dissociation curve (i.e., melting profile) for each probe-target duplex provided information on hybridization specificity, which is essential for confirming adequate discrimination between target and nontarget sequences.
    Keywords: Life Sciences (General)
    Type: Applied and environmental microbiology (ISSN 0099-2240); Volume 69; 4; 2377-82
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  • 8
    Publication Date: 2011-08-24
    Description: The discrimination between perfect-match and single-base-pair-mismatched nucleic acid duplexes was investigated by using oligonucleotide DNA microarrays and nonequilibrium dissociation rates (melting profiles). DNA and RNA versions of two synthetic targets corresponding to the 16S rRNA sequences of Staphylococcus epidermidis (38 nucleotides) and Nitrosomonas eutropha (39 nucleotides) were hybridized to perfect-match probes (18-mer and 19-mer) and to a set of probes having all possible single-base-pair mismatches. The melting profiles of all probe-target duplexes were determined in parallel by using an imposed temperature step gradient. We derived an optimum wash temperature for each probe and target by using a simple formula to calculate a discrimination index for each temperature of the step gradient. This optimum corresponded to the output of an independent analysis using a customized neural network program. These results together provide an experimental and analytical framework for optimizing mismatch discrimination among all probes on a DNA microarray.
    Keywords: Life Sciences (General)
    Type: Applied and environmental microbiology (ISSN 0099-2240); Volume 69; 5; 2848-56
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  • 9
    Publication Date: 2011-08-24
    Description: The effects of single-base-pair near-terminal and terminal mismatches on the dissociation temperature (T(d)) and signal intensity of short DNA duplexes were determined by using oligonucleotide microarrays and neural network (NN) analyses. Two perfect-match probes and 29 probes having a single-base-pair mismatch at positions 1 to 5 from the 5' terminus of the probe were designed to target one of two short sequences representing 16S rRNA. Nonequilibrium dissociation rates (i.e., melting profiles) of all probe-target duplexes were determined simultaneously. Analysis of variance revealed that position of the mismatch, type of mismatch, and formamide concentration significantly affected the T(d) and signal intensity. Increasing the concentration of formamide in the washing buffer decreased the T(d) and signal intensity, and it decreased the variability of the signal. Although T(d)s of probe-target duplexes with mismatches in the first or second position were not significantly different from one another, duplexes with mismatches in the third to fifth positions had significantly lower T(d)s than those with mismatches in the first or second position. The trained NNs predicted the T(d) with high accuracies (R(2) = 0.93). However, the NNs predicted the signal intensity only moderately accurately (R(2) = 0.67), presumably due to increased noise in the signal intensity at low formamide concentrations. Sensitivity analysis revealed that the concentration of formamide explained most (75%) of the variability in T(d)s, followed by position of the mismatch (19%) and type of mismatch (6%). The results suggest that position of the mismatch at or near the 5' terminus plays a greater role in determining the T(d) and signal intensity of duplexes than the type of mismatch.
    Keywords: Life Sciences (General)
    Type: Applied and environmental microbiology (ISSN 0099-2240); Volume 68; 1; 235-44
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  • 10
    Publication Date: 2019-08-15
    Description: Substance P inhibits renin secretion. This polypeptide is a transmitter in primary afferent neurons and is released from the peripheral as well as the central portions of these neurons. It is present in afferent nerves from the kidneys. Neuropeptide Y, which is a cotransmitter with norepinephrine and epinephrine, is found in sympathetic neurons that are closely associated with and presumably innervate the juxtagolmerular cells. Its effect on renin secretion is unknown, but it produces renal vasoconstriction and natriuresis. Vasoactive intestinal polypeptide (VIP) is a cotransmitter with acetylocholine in cholinergic neurons, and this polypeptide stimulates renin secretion. We cannot find any evidence for its occurence in neurons in the kidneys, but various stimuli increase plasma VIP to levels comparable to those produced by doses of exogenous VIP which stimulated renin secretion. Neostigmine increases plasma VIP and plasma renin activity, and the VIP appears to be responsible for the increase in renin secretion, since the increase is not blocked by renal denervation or propranolol. Stimulation of various areas in the brain produces sympathetically mediated increases in plasma renin activity associated with increases in blood pressure. However, there is pharmacological evidence that the renin response can be separated from the blood pressure response. In anaesthetized dogs, drugs that increase central serotonergic discharge increase renin secretion without increasing blood pressure. In rats, activation of sertonergic neurons in the dorsal raphe nucleus increases renin secretion by a pathway that projects from this nucleus to the ventral hypothalamus, and from there to the kidneys via the sympathetic nervous system. The serotonin releasing drug parachloramphetamine also increases plasma VIP, but VIP does not appear to be the primary mediator of the renin response. There is preliminary evidence that the serotonergic neurons in the dorsal raphe nucleus are part of the pathway by which psychosocial stimuli increase renin secretion.
    Keywords: Life Sciences (General)
    Type: Journal of hypertension (ISSN 0263-6352); 2 Suppl 1; 75-82
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