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  • 1
    ISSN: 0006-3592
    Keywords: methanol sensor ; methanol monitoring and control ; methylotrophic yeast fermentation ; Pichia pastoris ; transferrin ; shake-flask cultures ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The methylotrophic yeast Pichia pastoris can be used to express recombinant genes at high levels under the control of the methanol-inducible alcohol oxidase 1 (AOX1) promoter. Accurate regulation of the methanol concentration in P. pastoris cultures is necessary to maintain induction, while preventing accumulation of methanol to cytotoxic levels. We developed an inexpensive methanol sensor that uses a gas-permeable silicone rubber tube immersed in the culture medium and an organic solvent vapor detector. The sensor was used to monitor methanol concentration continuously throughout a fed-batch shake-flask culture of a P. pastoris clone producing the N-lobe of human transferrin. The sensor calibration was stable for the duration of the culture and the output signal accurately reflected the methanol concentration determined off-line by HPLC. A closed-loop control system utilizing this sensor was developed and used to maintain a 0.3% (v/v) methanol concentration in the culture. Use of this system resulted in a fivefold increase in volumetric protein productivity over levels obtained using the conventional fed-batch protocol. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 56: 279-286, 1997.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 27 (1985), S. 1662-1667 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The influence of bulk-water bacterial cell concentration and specific growth rate history on bacterial adsorption rates to surfaces was investigated using response surface analysis. A pure culture of Pseudomonas sp. 224S was grown in a chemostat and pumped into a continuous flow reactor where the bacteria were exposed to clean, glass surfaces under turbulent flow conditions for a period of six hours. Adsorption rate decreased approximately linearly with increasing specific growth rate history. Glass surfaces became saturated with 224S at ca. 0.1% coverage and the resulting spatial pattern of the adsorbed cells deviated from random in the direction of uniformity.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 24 (1982), S. 2115-2121 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 26 (1984), S. 1418-1424 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Aerobic glucose metabolism by Pseudomonas aeruginosa in steady-state biofilms at various substrate loading rates and reactor dilution rates was investigated. Variables monitored were substrate (glucose), biofilm cellular density, biofilm extracellular polymeric substance (EPS) density, and suspended cellular and EPS concentrations. A mathematical model developed to describe the system was compared to experimental data. Intrinsic yield and rate coefficients included in the model were obtained from suspended continuous culture studies of glucose metabolism by P. aeruginosa. Experimental data compared well with the mathematical model, suggesting that P. aeruginosa does not behave differently in steady-state biofilm cultures, where diffusional resistance is negligible, than in suspended cultures. This implies that kinetic and stoichiometric coefficients for P. aeruginosa derived in suspended continuous culture can be used to describe steady-state biofilm processes.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Publication Date: 2006-06-13
    Description: A continuing effort to develop a comprehensive capability for thermal-structural analysis and automated design (sizing) is reviewed. A principal role in the activity is played by the finite element program SPAR which contains both an efficient structural and thermal analysis capability. The benefit of having thermal and structural analyses in the same finite element program is illustrated by the application of SPAR to design calculations for the National Transonic Facility - a cryogenic wind tunnel under construction at Langley. Some experience with large-scale thermal structural analysis problems, particularly the space shuttle orbiter, has led to the identification of some analysis needs. Those needs include automated model generation and data output for lumped parameter thermal analysis, faster solution methods for nonlinear transient heat transfer, automated interpolation of temperature data from a thermal finite element model to a dissimilar structural finite model, and automated techniques to identify the times at which the critical combinations of transient heating and loads occur on a structure.
    Keywords: STRUCTURAL MECHANICS
    Type: Recent Advan. in Structures for Hypersonic Flight, Pt.2; p 897-941
    Format: text
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  • 6
    Publication Date: 2011-08-16
    Description: Investigation of the effect of using plane quadrilateral membrane elements for modeling nonplanar structures. The effect is assessed by analyzing a simplified finite element model with the aid of the structure network analysis program. The results obtained indicate that the use of planar quadrilateral membrane elements for modeling bending problems can lead to large errors if the four points that define the quadrilateral are not in the same plane.
    Keywords: STRUCTURAL MECHANICS
    Type: AIAA Journal; 11; May 1973
    Format: text
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  • 7
    Publication Date: 2016-06-07
    Description: An analytical study of a two-lobed circular arc pressure vessel (double-bubble tank) with a local perturbation from the cylindrical shape was made. It can be concluded that NASTRAN provides a good linear buckling analysis capability for structures that cannot be handled by other analysis techniques. However, solutions are relatively expensive from a computational standpoint. Two worthwhile additions to the NASTRAN program would be the inclusion of a new beam element that adequately represents the shear transfer when modeling stiffeners on a shell and the capability, in a rigid format, to keep certain prescribed loads constant during the eigenvalue extraction process.
    Keywords: STRUCTURAL MECHANICS
    Type: NASTRAN: Users' Experiences; p 53-71
    Format: application/pdf
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  • 8
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    In:  Other Sources
    Publication Date: 2019-05-30
    Description: Data from ground photography of Mars covering portions of Mariner IV path, noting position of oases and canals
    Keywords: SPACE SCIENCES
    Type: ICARUS
    Format: text
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  • 9
    Publication Date: 2014-09-03
    Description: Comparison of ASKA and NASTRAN programs analyses of space shuttle hot elevon test article deformed under thermal and mechanical loads
    Keywords: STRUCTURAL MECHANICS
    Type: NASA. Langley Res. Center NASTRAN: Users Experiences; p 163-180
    Format: text
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  • 10
    Publication Date: 2019-05-24
    Description: Mars surface as seen by Mariner IV photographs
    Keywords: SPACE SCIENCES
    Type: NASA-CR-64466 , TN-701-66-10
    Format: application/pdf
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