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  • 1
    Electronic Resource
    Electronic Resource
    Microsatellite repeats in grapevine reveal DNA polymorphisms when analysed as sequence-tagged sites (STSs) (1993)
    Springer
    Theoretical and applied genetics 86 (1993), S. 985-990 
    Details
    ISSN: 1432-2242
    Keywords: Grapevine ; Microsatellite DNA ; DNA polymorphisms ; STS ; SSLP ; STMS ; Vitis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Microsatellite repeat sequences were investigated as sequenced-tagged site (STS) DNA markers to determine the potential for genetic analysis of the grapevine genome. The PCR-generated markers detect codominant alleles at a single locus or site in the genome. The marker type is very informative detecting high heterozygosity (69%–88%) within individual grapevine cultivars and high genetic variation between cultivars, making it a useful marker type for plant genome mapping and genome typing. For five loci a screening of 26 V. vinifera cultivars found 13, 12, 8, 5, and 4 different length alleles respectively with some alleles more common than others. The genomic DNA sequences surrounding microsatellite sequences were conserved within the genus permitting STS primers to amplify STSs from other Vitis species. These Vitis species were found to have some unique alleles not present in V. vinifera.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00211051
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  • 2
    Electronic Resource
    Electronic Resource
    Integration of trinucleotide microsatellites into a linkage map of Citrus (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 701-706 
    Details
    ISSN: 1432-2242
    Keywords: Key words Citrus ; Microsatellites ; STS markers ; SSLP ; SSR ; Null alleles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We report the successful assignment of the first seven microsatellite markers to the Citrus RFLP and isozyme map. A total of 14 microsatellite primer pairs were developed and tested for amplification and product-length polymorphism within a population of plants previously used for linkage-map construction. In each case, the successfully assigned microsatellite mapped to the termini of a different linkage group indicating a widespread distribution throughout the genome. Analysis of allele segregation revealed that two of nine microsatellites displayed a significant deviation from expected ratios (P〉0.5). This was compared with other marker types within Citrus and a similar proportion of skewed loci was also found to be present. The analysis of two markers was complicated by the non-amplification of an inherited null allele within the mapping population. The successful integration of microsatellites into the genetic map of Citrus demonstrates the utility of this marker type for genetic analysis within wide intergeneric plant crosses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050468
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