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  • 1
    Electronic Resource
    Electronic Resource
    On-line monitoring and control of methanol concentration in shake-flask cultures of Pichia pastoris (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 56 (1997), S. 279-286 
    Details
    ISSN: 0006-3592
    Keywords: methanol sensor ; methanol monitoring and control ; methylotrophic yeast fermentation ; Pichia pastoris ; transferrin ; shake-flask cultures ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The methylotrophic yeast Pichia pastoris can be used to express recombinant genes at high levels under the control of the methanol-inducible alcohol oxidase 1 (AOX1) promoter. Accurate regulation of the methanol concentration in P. pastoris cultures is necessary to maintain induction, while preventing accumulation of methanol to cytotoxic levels. We developed an inexpensive methanol sensor that uses a gas-permeable silicone rubber tube immersed in the culture medium and an organic solvent vapor detector. The sensor was used to monitor methanol concentration continuously throughout a fed-batch shake-flask culture of a P. pastoris clone producing the N-lobe of human transferrin. The sensor calibration was stable for the duration of the culture and the output signal accurately reflected the methanol concentration determined off-line by HPLC. A closed-loop control system utilizing this sensor was developed and used to maintain a 0.3% (v/v) methanol concentration in the culture. Use of this system resulted in a fivefold increase in volumetric protein productivity over levels obtained using the conventional fed-batch protocol. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 56: 279-286, 1997.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 2
    Electronic Resource
    Electronic Resource
    Bacterial adsorption to smooth surfaces: Rate, extent, and spatial pattern (1985)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 27 (1985), S. 1662-1667 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The influence of bulk-water bacterial cell concentration and specific growth rate history on bacterial adsorption rates to surfaces was investigated using response surface analysis. A pure culture of Pseudomonas sp. 224S was grown in a chemostat and pumped into a continuous flow reactor where the bacteria were exposed to clean, glass surfaces under turbulent flow conditions for a period of six hours. Adsorption rate decreased approximately linearly with increasing specific growth rate history. Glass surfaces became saturated with 224S at ca. 0.1% coverage and the resulting spatial pattern of the adsorbed cells deviated from random in the direction of uniformity.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260271206
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  • 3
    Electronic Resource
    Electronic Resource
    Microbial desulfurization of coal by thermophilic microorganism Sulfolobus acidocaldarius (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 24 (1982), S. 2115-2121 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260240919
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  • 4
    Electronic Resource
    Electronic Resource
    Activity of Pseudomonas aeruginosa in biofilms: Steady state (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 26 (1984), S. 1418-1424 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Aerobic glucose metabolism by Pseudomonas aeruginosa in steady-state biofilms at various substrate loading rates and reactor dilution rates was investigated. Variables monitored were substrate (glucose), biofilm cellular density, biofilm extracellular polymeric substance (EPS) density, and suspended cellular and EPS concentrations. A mathematical model developed to describe the system was compared to experimental data. Intrinsic yield and rate coefficients included in the model were obtained from suspended continuous culture studies of glucose metabolism by P. aeruginosa. Experimental data compared well with the mathematical model, suggesting that P. aeruginosa does not behave differently in steady-state biofilm cultures, where diffusional resistance is negligible, than in suspended cultures. This implies that kinetic and stoichiometric coefficients for P. aeruginosa derived in suspended continuous culture can be used to describe steady-state biofilm processes.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260261204
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  • 5
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    Teflon, Kapton surface and bulk effects (1985)
    In:  CASI
    Details
    Publication Date: 2006-02-14
    Description: Two samples of 2 mil aluminized/Kapton thermal blanket and two samples of Teflon were received for evaluation. Their location on the solar maximum is given. The Kapton top layer of the thermal blanket had been exposed to the LEO atomic oxygen environment and shows the surface degradation due to atomic oxygen attack resulting in a diffuse character over most of the surface. The backside Kapton layer which was unexposed appeared to the eye to be in virgin condition. Another sample exhibited similar properties, but was, in large part, covered with Kapton adhesive tape and it was not possible to obtain usable specimens for analysis. One of the Teflon samples which was exposed shows signs of heavy degradation including attack on the Ag/Inconel backside by ultraviolet and atomic oxygen. The other Teflon sample which was unexposed was only slightly fogged on the Teflon side and the Ag/Inconel appeared untouched. These samples were subjected to several chemical and physical analyses, the results of which are discussed.
    Keywords: SPACECRAFT DESIGN, TESTING AND PERFORMANCE
    Type: NASA. Goddard Space Flight Center Proceedings of the SMRM Degradation Study Workshop; p 287-316
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    NASA TECHNICAL REPORTS
  • 6
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    External tank space debris considerations (1992)
    In:  Other Sources
    Details
    Publication Date: 2019-06-28
    Description: Orbital debris issues associated with maintaining a Space Shuttle External Tank (ET) on orbit are presented. The first issue is to ensure that the ET does not become a danger to other spacecraft by generating space debris, and the second is to protect the pressurized ET from penetration by space debris or meteoroids. Tests on shield designs for penetration resistance showed that when utilized with an adequate bumper, thermal protection system foam on the ET is effective in preventing penetration.
    Keywords: SPACECRAFT DESIGN, TESTING AND PERFORMANCE
    Type: AIAA PAPER 92-1411
    Format: text
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    NASA TECHNICAL REPORTS
  • 7
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    TSS tether cable meteoroid/orbital debris damage analysis (1993)
    In:  CASI
    Details
    Publication Date: 2019-06-28
    Description: This report summarizes the damage analyses performed on the tether cable used for the tethered satellite system (TSS), for the damage that could be caused by meteoroid or orbital debris impacts. The TSS consists of a tethered satellite deployer and a tethered satellite. The analytical studies were performed at Marshall Space Flight Center (MSFC) with the results from the following tests: (1) hypervelocity impact tests to determine the 'critical' meteoroid particle diameter, i.e., the maximum size of a meteoroid particle which can impact the tether cable without causing 'failure'; (2) electrical resistance tests on the damaged and undamaged tether cable to determine if degradation of current flow occurred through the damaged tether cables; and (3) tensile load tests to verify the load carrying capability of the damaged tether cables. Finally, the HULL hydrodynamic computer code was used to simulate the hypervelocity impact of the tether cable by particles at velocities higher than can be tested, to determine the extent of the expected tether damage.
    Keywords: SPACECRAFT DESIGN, TESTING AND PERFORMANCE
    Type: NASA-TM-108404 , NAS 1.15:108404
    Format: application/pdf
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    NASA TECHNICAL REPORTS
  • 8
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    Preliminary design of a meteoroid/orbital debris shield system for a Mars mission spacecraft (1992)
    In:  Other Sources
    Details
    Publication Date: 2019-06-28
    Description: A preliminary design of a spacecraft shield system to defeat meteoroids and orbital debris during a Mars mission is presented. The mission scenario is first defined in terms of stage times which include LEO, transit, low-Mars orbit, and Mars surface periods. The environment definitions for earth-orbital space debris, planetary meteoroids, and interplanetary meteoroids are next introduced. Shield system design approaches incorporate stochastic simulation, hydrocode analyses, hypervelocity impact testing, and optimization techniques. Structural design trades presented include spacecraft configuration, mission schedule, penetration risk, and total standoff distance between the bumper and wall.
    Keywords: SPACECRAFT DESIGN, TESTING AND PERFORMANCE
    Type: AIAA PAPER 92-1406
    Format: text
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  • 9
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    Some aspects of algorithm performance and modeling in transient thermal analysis of structures (1981)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Description: The status of an effort to increase the efficiency of calculating transient temperature fields in complex aerospace vehicle structures is described. The advantages and disadvantages of explicit and implicit algorithms are discussed. A promising set of implicit algorithms with variable time steps, known as the GEAR package is described. Four test problems, used for evaluating and comparing various algorithms, have been selected and finite-element models of the configurations are described. These problems include a Space Shuttle frame component, an insulated cylinder, a metallic panel for a thermal protection system, and a model of the Space Shuttle Orbiter wing. Results generally indicate a preference for implicit over explicit algorithms for solution of transient structural heat transfer problems when the governing equations are 'stiff' (typical of many practical problems such as insulated metal structures).
    Keywords: SPACECRAFT DESIGN, TESTING AND PERFORMANCE
    Type: ASME PAPER 81-WA/HT-4 , Winter Annual Meeting; Nov 15, 1981 - Nov 20, 1981; Washington, DC
    Format: text
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  • 10
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    Electric fields and secondary emission near a dielectric-metal interface (1979)
    In:  Other Sources
    Details
    Publication Date: 2019-06-27
    Description: Dielectric surface-charge distributions near a metal-dielectric interface in vacuum depend upon secondary emission processes in the presence of normal and tangential components of electric field. From measured charge distributions created by exposing a specimen of fluorinated ethylene-propylene to monoenergetic electron fluxes, it has been possible to calculate potentials and fields on and near the dielectric surface. The effect of the normal electric field upon secondary emission is measured directly, and the effect of the tangential field is inferred from the charge-distribution data. The critical point (unity crossover) for secondary emission is shifted by the application of fields, so that it occurs at much higher primary energies than normally. Primary beams having energies up to 20 keV are used, and surface fields are as high as 20 kV/mm.
    Keywords: SPACECRAFT DESIGN, TESTING AND PERFORMANCE
    Type: IEEE Transactions on Electrical Insulation; EI-14; Feb. 197
    Format: text
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