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  • SOLAR PHYSICS  (4)
  • Escherichia coli  (3)
  • 1985-1989  (7)
  • 1935-1939
  • 1
    Electronic Resource
    Electronic Resource
    Effects of copper concentration in continuous culture on the aa 3-type cytochrome oxidase and respiratory chains of Paracoccus denitrificans (1989)
    Springer
    Archives of microbiology 151 (1989), S. 300-306 
    Details
    ISSN: 1432-072X
    Keywords: Copper-limitation ; Escherichia coli ; Cytochrome oxidases ; Oxygen reduction ; Respiratory chains
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The role(s) of copper in a bacterial cytochrome oxidase of the aa 3-type was investigated by growth of Paracoccus denitrificans NCIB 8944, in batch and steady state continuous culture, in a medium from which the bulk of the copper had been extracted. In a medium containing approximately 0.02 μM copper, cellular copper content, cytochromes a+a 3 and cytochrome a 3 were reduced to 55%, 58% and 33% respectively of control values and there were also less marked decreases in cytochromes c+c 1 (to 85%) and a CO-binding b-type cytochrome, possibly cytochrome o (to 71%). Copper deficiency elicited in reduced minus oxidized difference spectra a shift to shorter wavelengths and narrowing of the band width of the α-band of the oxidase, and loss of a (negative) band near 830 nm attributable to CuA (the copper functionally associated with haem a in the oxidase complex). The oxidase in copper-deficient cells reacted with oxygen to form the oxy “Compound A” at rates similar to that in control cells but CO recombination to ferrous haem a 3 was slowed 4-fold in the copper deficient case. The results are interpreted as indicating loss of CuA and changes in the proportions of haems a and a 3 with retention of catalytic activity. Titrations of respiration rates with antimycin suggested that copper deficiency did not result in diversion of electron flux through an antimycin A-insensitive, cytochrome o-terminated branch of the respiratory chain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00406555
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Effects of iron-limitation ofEscherichia coli on growth, the respiratory chains and gallium uptake (1986)
    Springer
    Archives of microbiology 146 (1986), S. 80-86 
    Details
    ISSN: 1432-072X
    Keywords: Iron-limitation ; Escherichia coli ; Respiratory chains ; Cytochromes ; Gallium ; Metal uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of iron limitation on growth, the composition and function of the respiratory chains, and gallium uptake inEscherichia coli have been studied. Decreasing the iron concentration in a defined medium using Chelex resin gave lower growth yields in both continuous culture and prolonged batch culture. In the former, ironlimited (entering [Fe]≤2.0 μM) cells exhibited diminished respiration rates, respiration-driven proton translocation quotients, and levels of non-haem iron and cytochromes. The cellular concentration of haemoproteinb-590 (a cytochromea 1-like hydroperoxidase) decreased 20-fold on iron limitation, whilst a CO-binding pigment with an absorption maximum in the dithionite-treated form near 500 nm appeared. Gallium(III) (9 μM) added to iron-limited, but not iron-sufficient, cultures diminished growth yields further; cells grown with low entering concentrations of iron took up less gallium than iron-sufficient cells. These results are attributed to the interference by gallium(III) with siderophore-mediated metal uptake. Gallium also stimulated iron uptake and was itself accumulated by iron-sufficient cells, suggesting that gallium(III) also affects the iron transport system(s) of low affinity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690163
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  • 3
    Electronic Resource
    Electronic Resource
    Identification of the promotors directing in vivo expression of hemolysin genes in Proteus vulgaris and Escherichia coli (1988)
    Springer
    Molecular genetics and genomics 213 (1988), S. 99-104 
    Details
    ISSN: 1617-4623
    Keywords: Promoters ; Escherichia coli ; Proteus vulgaris ; Hemolysin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The hemolytic activity of Escherichia coli and Proteus vulgaris is determined by common contiguous genes encoding synthesis (hly C, hly A) and specific secretion (hly B, hly D) of active hemolysin. Nevertheless, the hlyC-proximal DNA sequences directing production of the homologous hemolysins by the recombinant DNAs P. vulgaris pVU763-709 and E. coli pANN202-312 showed no extensive homology. Primer extension and S1 nuclease protection were used to define in the two sequences the 5′ termini of hly transcripts synthesized in vivo and thus to infer the active hly promoters sequences. The E. coli hly C upstream region contained three separate promotors directing in vivo hly transcription, while the corresponding transcription of the P. vulgaris hly operon originated from a single distinct promotor, the-35 and-10 sequences of which formed part of an inverted repeat sequence. Elevated hemolytic activity caused by upstream Tn5 insertions in pVU763-709 resulted from increased transcription from this promotor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00333404
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  • 4
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    The 10.7-cm microwave observations of AR 5395 and related terrestrial effects (1989)
    In:  CASI
    Details
    Publication Date: 2013-08-31
    Description: The 10.7 cm flux patrols in Canada recorded 4 Great Bursts (peaks greater than 500 sfu) during the disk passage of AR 5395 in March 1989. The Great Bursts of 16 and 17 March were simple events of great amplitude and with half-life durations of only several minutes. Earlier Great Bursts, originating on 6 March towards the NE limb and on 10 March closer to the central meridian, belong to an entirely different category of event. Each started with a very strong impulsive event lasting just minutes. After an initial recovery, however, the emission climbed back to level as greater or greater than the initial impulsive burst. The events of 6 and 10 March stayed above the Great Burst threshold for at least 100 minutes. The second component of long duration in these cases is associated with Type 4 continuum emission and thus very likely with CMEs. Major geomagnetic disturbances did not occur as a result of the massive complex event of 6 March or the two simple but strong events of 16 and 17 March. But some 55 hours after the peak in the long-enduring burst of 10 March, a storm began which qualifies as the fourth strongest geomagnetic storm in Canada since 1932. The vertical component of the earth's field measured during the storm by a fluxgate magnetometer at a station in Manitoba is presented. Within a minute of the sudden commencement of this storm, a series of breakdowns began in the transmission system of Hydro-Quebec which resulted in a total loss of power, on a bitterly cold winter's day, for at least 10 hours. The loss of power provoked an enormous outcry from the public resulting in the power utilities being more receptive to the need to monitor solar as well as geomagnetic activity.
    Keywords: SOLAR PHYSICS
    Type: NASA, Goddard Space Flight Center, Max '91 Workshop 2: Developments in Observations and Theory for Solar Cycle 22; p 242-245
    Format: application/pdf
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  • 5
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    Magnetic fields in the overshoot zone: The great escape (1987)
    In:  CASI
    Details
    Publication Date: 2019-06-28
    Description: In order that magnetic flux be confined within the solar interior for times comparable to the solar cycle period it has been suggested that the bulk of the solar toroidal field is stored in the convectively stable overshoot region situated beneath the convection zone proper. Such a magnetic field, though, is still buoyant and is therefore subject to Rayleigh-Taylor type instabilities. The model problem of an isolated region of magnetic field embedded in a convectively stable atmosphere is considered. The fully nonlinear evolution of the two dimensional interchange of modes is studied, thereby shedding some light on one of the processes responsible for the escape of flux from the solar interior.
    Keywords: SOLAR PHYSICS
    Type: NASA-Goddard Space Flight Center, Theoretical Problems in High Resolution Solar Physics, 2; p 101-104
    Format: application/pdf
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  • 6
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    The nonlinear breakup of a magnetic layer - Instability to interchange modes (1988)
    In:  Other Sources
    Details
    Publication Date: 2019-07-12
    Description: Motivated by considerations of the solar toroidal magnetic field, the behavior of a layer of uniform magnetic field embedded in a convectively stable atmosphere is studied. Since the field can support extra mass, such a configuration is top-heavy and thus instabilities of the Rayleigh-Taylor type can occur. For both static and rotating basic states, the evolution of the interchange modes (no bending of the field lines) is followed by integrating numerically the nonlinear compressible MHD equations. The initial Rayleigh-Taylor instability of the magnetic field gives rise to strong shearing motions, thereby exciting secondary Kelvin-Helmholtz instabilities which wrap the gas into regions of intense vorticity. The subsequent motions are determined primarily by the strong interactions between vortices which are responsible for the rapid disruption of the magnetic layer.
    Keywords: SOLAR PHYSICS
    Type: Journal of Fluid Mechanics (ISSN 0022-1120); 196; 323-344
    Format: text
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  • 7
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    The nonlinear breakup of the sun's toroidal field (1989)
    In:  Other Sources
    Details
    Publication Date: 2019-07-13
    Description: There are good reasons for believing that the sun has a strong toroidal magnetic field in the stably stratified region of convective overshoot sandwiched between the radiative zone and convective zone proper. The magnetic field in this region is modeled by studying the behavior of a layer of uniform field embedded in a subadiabatic atmosphere. Since the field can support extra mass, such a configuration is top-heavy, and instabilities of the Rayleigh-Taylor type can occur. Numerical integration of the two-dimensional compressible MHD equations makes it possible to follow the evolution of this instability into the nonlinear regime. The initial buoyancy-driven instability of the magnetic field gives rise to strong shearing motions, thereby exciting secondary Kelvin-Helmholtz instabilities which wrap the gas into regions of intense vorticity. The somewhat surprising subsequent motions are determined primarily by the strong interactions between vortices.
    Keywords: SOLAR PHYSICS
    Type: European Physical Society Study Conference; Jun 16, 1988 - Jun 21, 1988; Noto; Italy
    Format: text
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