ISSN:
1573-4927
Keywords:
DROSOPHILA
;
SN-GLYCEROL-3-PHOSPHATE DEHYDROGENASE
;
ENHANCER
;
GPDH
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract P element-mediated transformation has been usedto investigate the regulation of expression of thesn-glycerol-3-phosphate dehydrogenase gene ofDrosophila melanogaster. A 13-kb constructcontaining the eight exons and associated introns, 5 kb of the5′ region, and 3 kb downstream from the structuralgene produced normal levels of enzyme activity andrescued the poor viability of flies lacking the enzyme. All the regulatory elements essential fornormal enzyme expression were located in a fragment thatincluded the exons and introns and 1-kb upstreamnoncoding sequence. Deletions of the 1.6-kb secondintron reduced activity to 25%. Transformants withfusion constructs between the sn-glycerol-3-phosphatedehydrogenase gene and the beta-galactosidase gene fromE. coli revealed three elements that affectedexpression. A (CT)9 repeat element at the5′ end of the second intron increased expressionin both larvae and adults, particularly at emergence. Asecond regulatory element, which includes a(CT)7 repeat, was located 5′ to the TATA box and had similareffects on the gene's expression. A third, undefined,enhancer was located in the second intron, between 0.5and 1.8 kb downstream of the translation initiationcodon. This element increases enzyme activity to asimilar extent in larvae and adults but has littleeffect when the enhancer at the 5′ end of theintron is present.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1018745412966
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