Publication Date:
2007-04-28
Description:
One component of the circadian clock in mammals is the Clock-Bmal1 heterodimeric transcription factor. Among its downstream targets, two genes, Cry1 and Cry2, encode inhibitors of the Clock-Bmal1 complex that establish a negative-feedback loop. We found that both Cry1 and Cry2 proteins are ubiquitinated and degraded via the SCF(Fbxl3) ubiquitin ligase complex. This regulation by SCF(Fbxl3) is a prerequisite for the efficient and timely reactivation of Clock-Bmal1 and the consequent expression of Per1 and Per2, two regulators of the circadian clock that display tumor suppressor activity. Silencing of Fbxl3 produced no effect in Cry1-/-;Cry2-/- cells, which shows that Fbxl3 controls clock oscillations by mediating the degradation of CRY proteins.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Busino, Luca -- Bassermann, Florian -- Maiolica, Alessio -- Lee, Choogon -- Nolan, Patrick M -- Godinho, Sofia I H -- Draetta, Giulio F -- Pagano, Michele -- MC_U142684172/Medical Research Council/United Kingdom -- MC_U142684173/Medical Research Council/United Kingdom -- MC_U142684175/Medical Research Council/United Kingdom -- R01-GM57587/GM/NIGMS NIH HHS/ -- R21-CA125173/CA/NCI NIH HHS/ -- R37-CA76584/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 2007 May 11;316(5826):900-4. Epub 2007 Apr 26.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pathology, NYU Cancer Institute, New York University School of Medicine, 550 First Avenue, MSB 599, New York, NY 10016, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17463251" target="_blank"〉PubMed〈/a〉
Keywords:
ARNTL Transcription Factors
;
Animals
;
Basic Helix-Loop-Helix Transcription Factors/metabolism
;
CLOCK Proteins
;
Cell Cycle Proteins/genetics/metabolism
;
Cells, Cultured
;
*Circadian Rhythm/genetics
;
Cryptochromes
;
F-Box Proteins/genetics/*metabolism
;
Flavoproteins/genetics/*metabolism
;
HeLa Cells
;
Humans
;
Mice
;
NIH 3T3 Cells
;
Nuclear Proteins/genetics/metabolism
;
Period Circadian Proteins
;
Promoter Regions, Genetic
;
RNA Interference
;
SKP Cullin F-Box Protein Ligases/*metabolism
;
Trans-Activators/metabolism
;
Transcription Factors/genetics/metabolism
;
Transfection
;
Ubiquitin/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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