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  • Puerto Rico  (2)
  • S-100 protein  (2)
  • Springer  (4)
  • 1
    Electronic Resource
    Electronic Resource
    Reproduction in an aggregating grouper, the red hind,epinephelus guttatus (1994)
    Springer
    Environmental biology of fishes 41 (1994), S. 269-286 
    Details
    ISSN: 1573-5133
    Keywords: Spawning ; Serranidae ; Pisces ; Puerto Rico ; Protogyny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Synopsis We examined the reproductive pattern of an aggregating grouper, the red hind,Epinephelus guttatus, in Puerto Rico. Macroscopic and histological examination of gonads confirmed that, although mature, ripe ovaries are found over a three-month period, spawning activity is limited to about 2 weeks each year. Females are determinate spawners and individuals are able to spawn more than once during the course of the annual spawning season. The red hind is protogynous and 50% maturity is attained at 215 mm fork length. In western Puerto Rico, spawning occurs in aggregations at several sites within loosely defined areas located towards the edge of the insular platform. Sex ratios of individuals taken by hook and line at one of the aggregation areas over a consecutive six-year period, suggest considerable intra- and inter-annual variation most likely attributable to a combination of differential ingress and egress by males and females during the course of an aggregation and to fluctuations in recruitment, differential mortality by sex and sex change among years. A comparative assessment of the reproductive patterns of seven western AtlanticEpinephelus spp. suggests that aggregation-spawning is associated with medium- to large-sized groupers, while smaller groupers do not aggregate. Mating systems vary among congeners in association with trends in male-female size dimorphism, sexual pattern and sperm competition. The short-term, localized, nature of spawning aggregations renders species with this reproductive mode particularly vulnerable to fishing pressure at spawning sites. Aggregating species, therefore, may require special management consideration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02197849
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  • 2
    Electronic Resource
    Electronic Resource
    Reproduction in an aggregating grouper, the red hind,epinephelus guttatus (1994)
    Springer
    Environmental biology of fishes 41 (1994), S. 269-286 
    Details
    ISSN: 1573-5133
    Keywords: Spawning ; Serranidae ; Pisces ; Puerto Rico ; Protogyny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Synopsis We examined the reproductive pattern of an aggregating grouper, the red hind,Epinephelus guttatus, in Puerto Rico. Macroscopic and histological examination of gonads confirmed that, although mature, ripe ovaries are found over a three-month period, spawning activity is limited to about 2 weeks each year. Females are determinate spawners and individuals are able to spawn more than once during the course of the annual spawning season. The red hind is protogynous and 50% maturity is attained at 215 mm fork length. In western Puerto Rico, spawning occurs in aggregations at several sites within loosely defined areas located towards the edge of the insular platform. Sex ratios of individuals taken by hook and line at one of the aggregation areas over a consecutive six-year period, suggest considerable intra- and inter-annual variation most likely attributable to a combination of differential ingress and egress by males and females during the course of an aggregation and to fluctuations in recruitment, differential mortality by sex and sex change among years. A comparative assessment of the reproductive patterns of seven western AtlanticEpinephelus spp. suggests that aggregation-spawning is associated with medium- to large-sized groupers, while smaller groupers do not aggregate. Mating systems vary among congeners in association with trends in male-female size dimorphism, sexual pattern and sperm competition. The short-term, localized, nature of spawning aggregations renders species with this reproductive mode particularly vulnerable to fishing pressure at spawning sites. Aggregating species, therefore, may require special management consideration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023817
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of S-100 protein binding sites in synaptosomal fractions and subfractions (1982)
    Springer
    Cellular and molecular neurobiology 2 (1982), S. 265-276 
    Details
    ISSN: 1573-6830
    Keywords: S-100 protein ; binding sites ; synaptosomes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. Immunocytochemical evidence is presented of the ultrastructural localization of binding sites for S-100 protein in synaptosomal fractions and subfractions. Synaptosomes or isolated synaptosomal subfractions were first incubated with S-100, then centrifuged to remove unbound S-100, and finally fixed before treatment with anti-S-100 antiserum, using the unlabeled antibody peroxidase-antiperoxidase (PAP) method. 2. When intact synaptosomes were used, the immunoreaction product was localized to the postsynaptic density including the postsynaptic membrane. In some reactive synaptosomes, the presynaptic membrane was labeled as well, in the region of synaptic contact. No reaction deposit was found in the synaptic cleft or on intrasynaptosomal structures. Divalent cations (Ca2+ and Mg2+) were essential for S-100 to interact with synaptosomes. Of the synaptosomal subfractions tested, i.e., synaptic vesicles and intraterminal mitochondria, only synaptic vesicles showed immunoreactivity when treated with S-100 and anti-S-100 antiserum as described above. 3. The S-100 immunoreactivity in synaptic structures documented in this report parallels the distribution of the high-affinity binding sites for radiolabeled S-100 in synaptosomal fractions and subfractions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00710848
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  • 4
    Electronic Resource
    Electronic Resource
    Biochemical and physicochemical properties of the solubilized S-100 protein binding activity of synaptosomal particulate fractions (1983)
    Springer
    Cellular and molecular neurobiology 3 (1983), S. 239-254 
    Details
    ISSN: 1573-6830
    Keywords: S-100 protein ; synaptosomes ; S-100 binding sites ; Ca2+-binding protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. The125I-labeled S-100 specific binding to a Triton X-100 (TX-100) extract of synaptosomal particulate fractions (SYN) was investigated. 2. The results indicate that (a) S-100 binding to the TX-100 extract is partially irreversible after a critical association time at 37°C, while it is fully reversible after any association time at 4°C; (b) trypsin and phospholipase C partially reverse the S-100 binding, while phospholipase D enhances the interaction to some extent, in a dose-dependent way; (c) EDTA and high concentrations of NaC1 or KC1 are more efficient as inhibitors of the S-100 binding to the TX-100 extract than as125I-labeled S-100 dissociating agents, in analogy with previous observations with SYN; and (d) two main populations of solubilized S-100 binding sites can be evidenced by gel filtration and sucrose gradient centrifugation when low amounts of the TX-100 extract are processed and/or low S-100 concentrations are used, while two additional molecular species are separated when greater amounts of either factors are tested. 3. These results suggest the possibility that S-100 may be involved in the regulation of some membrane activities.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00710950
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