ISSN:
1617-4623
Keywords:
Agrobacterium rhizogenes
;
Root induction
;
T-DNA
;
Transformation
;
Tobacco
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Disarmed plant transformation vectors were used to assay the ability of subfragments of the T-regions of the Ri plasmid of agropine-type strain A4 of Agrobacterium rhizogenes to induce proliferation of transformed roots on tobacco leaf fragments. We have shown that a 6 kb region of TR-DNA, bearing the presumptive auxin synthesis genes, is capable of inducing transformed roots with an essentially normal phenotype as had been shown previously with the entire TR-region. A 1.9 kb fragment of the 20 kb TL-region is suffcient to induced transformed roots in the absence of exogenous hormones. These roots grow profusely on hormone-free medium, as is typical of roots transformed by the intact TL-DNA.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00337765
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