ISSN:
1432-2242
Keywords:
Rice
;
Adenine methylation
;
Tissue specificity
;
Genomic rearrangement
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Using ‘A’ and ‘C’ methylation-specific restriction enzymes, namely, MboI, Sau3AI, DpnI, MspI, and HpaII, total rice cv Basmati 370 DNA, repetitive DNAs, and a specific repeat sequence indicated an abundance of adenine methylation. Although cytosine methylation in 5′-CCGG-3′ sequences suggested more CpC methylation than CpG, the ‘C’ methylation in sequence 5′-GATC-3′ was comparatively less than ‘A’ methylation. Furthermore, the presence of adenine methylation was tissue specific; it was predominant in rice shoot DNA as compared to embryo DNA. This pattern was also observed in two other cultivars of rice, i.e., R-24 and Sona, and was again confirmed using a cloned probe of a specific repeat sequence. Besides the changes in adenine methylation, there was also a qualitative change in 5mC from CpG to CpC dinucleotides in these two tissue systems.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00210080
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