ISSN:
1617-4623
Keywords:
Yeast
;
Mitochondrial 15 S rRNA
;
Ribosomal frameshifting
;
Paromomycin resistance
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The leaky expression of the yeast mitochondrial geneoxi1, containing a frameshift mutation (+1), is caused by natural frameshift suppression, as shown previously (Fox and Weiss-Brummer 1980). A drastic decrease in the natural level of frameshifting is found in the presence of thepar r-454 mutation, localized at the 3′ end of the 15 S rRNA gene. This mutation causes resistance to the antibiotic paronomycin in the yeast strains D273-10B and KL14-4A (Li et al. 1982; Tabak et al. 1982). The results of this study imply that in the yeast strain 777-3A this mutation alone is sufficient for restriction of the level of natural frameshifting but is insufficient to confer resistance to paromomycin. A second mutation, arising spontaneously with a frequency of 10−4 leads, in combination with thepar r-454 mutation, to full paromomycin resistance in strain 777-3A.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02464905
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